![Western Blot: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody (M01), clone 1B7.Lane 1: F2 transfected lysate(70 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Coagulation-Factor-II-Thrombin-Antibody-1B7-Western-Blot-H00002147-M01-img0008.jpg "Western Blot: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody (M01), clone 1B7.Lane 1: F2 transfected lysate(70 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Func, IP, KD |
| Clone | 1B7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | F2 (AAH51332, 263 a.a. ~ 362 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RNPDGDEEGAWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDG |
| Localization | Secreted |
| Specificity | F2 - coagulation factor II (thrombin) |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | F2 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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Secondary Antibodies |
Isotype Controls |
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![Western Blot: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi ( Cat # H00002147-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with F2 monoclonal antibody (M01), clone 1B7 (Cat # H00002147-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Coagulation-Factor-II-Thrombin-Antibody-1B7-Western-Blot-H00002147-M01-img0006.jpg "Western Blot: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi ( Cat # H00002147-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with F2 monoclonal antibody (M01), clone 1B7 (Cat # H00002147-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Proximity Ligation Assay: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of protein-protein interactions between FGA and F2. HeLa cells were stained with anti-FGA rabbit purified polyclonal 1:1200 and anti-F2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Coagulation-Factor-II-Thrombin-Antibody-1B7-Proximity-Ligation-Assay-H00002147-M01-img0004.jpg "Proximity Ligation Assay: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of protein-protein interactions between FGA and F2. HeLa cells were stained with anti-FGA rabbit purified polyclonal 1:1200 and anti-F2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
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