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CNAP1 Antibody (4C12)

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Western Blot: CNAP1 Antibody (4C12) [H00009918-M01] - CNAP1 monoclonal antibody (M01), clone 4C12 Analysis of CNAP1 expression in HeLa.
Western Blot: CNAP1 Antibody (4C12) [H00009918-M01] - CNAP1 monoclonal antibody (M01), clone 4C12 Analysis of CNAP1 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: CNAP1 Antibody (4C12) [H00009918-M01] - Analysis of monoclonal antibody to CNAP1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: CNAP1 Antibody (4C12) [H00009918-M01] - Analysis of monoclonal antibody to CNAP1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
Western Blot: CNAP1 Antibody (4C12) [H00009918-M01] - Analysis of CNAP1 expression in transfected 293T cell line by CNAP1 monoclonal antibody (M01), clone 4C12.Lane 1: CNAP1 transfected lysate(157 KDa).Lane 2: ...read more
Sandwich ELISA: CNAP1 Antibody (4C12) [H00009918-M01] - Detection limit for recombinant GST tagged CNAP1 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
4C12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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CNAP1 Antibody (4C12) Summary

Immunogen
CNAP1 (AAH28182, 1240 a.a. ~ 1339 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LRKMLDNFDCFGDKLSDESIFSAFLSVVGKLRRGAKPEGKAIIDEFEQKLRACHTRGLDGIKELEIGQAGSQRAPSAKKPSSGSRYQPLASTASDNDFVT
Localization
Nuclear and cytoplasmic.
Specificity
CNAP1 - chromosome condensation-related SMC-associated protein 1
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NCAPD2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against recominant protein, transfected lysate and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CNAP1 Antibody (4C12)

  • CAPD2
  • CAP-D2
  • Chromosome condensation-related SMC-associated protein 1
  • Chromosome-associated protein D2
  • CNAP1XCAP-D2 homolog
  • hCAP-D2condensin complex subunit 1
  • KIAA0159chromosome condensation related SMC associated protein 1
  • Non-SMC condensin I complex subunit D2
  • non-SMC condensin I complex, subunit D2

Background

CNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NCAPD2
Entrez
Uniprot