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cIAP-2/HIAP-1 Antibody [Unconjugated]

 (17 Publications)
 
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cIAP‑2/HIAP‑1 was detected in immersion fixed paraffin-embedded sections of human colon tissue using Goat Anti-Human cIAP‑2/HIAP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to colon glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
cIAP‑2/HIAP‑1 was detected in immersion fixed paraffin-embedded sections of human colon tissue using Goat Anti-Human cIAP‑2/HIAP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) at 1 ...read more
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human cIAP-2/HIAP-1 Antigen Affinity-purified ...read more
Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP‑2/HIAP‑1 at approximately 70 ...read more
IAP expression in glioblastoma cell lines. Expression levels of cIAP1, cIAP2, XIAP and ML-IAP were analyzed by western blotting and quantified in U87MG and GL261 adherent GBM cell lines, and in GBM6 and GBM9 spheres. ...read more
Prognostic value of cIAP1, cIAP2, XIAP and ML-IAP protein expression in human glioblastomas (cohorts 1 and 2). (a) cIAP1-, cIAP2-, XIAP- and ML-IAP-positive stainings in GBM. IAPs were heterogeneously expressed by tumor ...read more
Apoptosis and IAP expression upon SMAC mimetic GDC-0152 treatment in glioblastoma cell lines. (a) Apoptosis (SubG0/G1) of DMSO control and GDC-0152-treated cells was determined by flow cytometry of propidium ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF8171
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF8171-SP
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cIAP-2/HIAP-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human cIAP‑2/HIAP‑1
Asn2-Ser604
Accession # U45878
Specificity
Detects human cIAP‑2/HIAP‑1 in Western blots. In Western blots, less than 1% cross-reactivity with cIAP-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BIRC3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF8171 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for cIAP-2/HIAP-1 Antibody [Unconjugated]

  • AIP1
  • API2
  • BIRC3 baculoviral IAP repeat containing 3
  • BIRC3
  • cIAP2
  • c-IAP2
  • cIAP-2
  • HAIP1
  • HIAP1
  • HIAP-1
  • MALT2
  • MIHC
  • RNF49

Background

cIAP-2 (also known as MIHC and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-2 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-2 inhibits caspases through the direct interaction of its BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HtrA2/Omi. cIAP-2 is reported to be cleaved by HtrA2/Omi.

  1. Roy, N. et al. (1997) EMBO J. 23:6914.
  2. Deveraux, Q. et al. (1997) Nature 388:300.
  3. Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
  4. Srinivasula, S.M. et al. (2003) J. Biol. Chem. 278:31469.
  5. Yang, Q-H. et al. (2003) Genes Dev. 17:1487.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for cIAP-2/HIAP-1 Antibody (AF8171)(17)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow Cytometry, ICC, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
Western Blot
(10)
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Human
(11)
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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF8171 Applications Species
Y An, J Jeon, L Sun, A Derakhshan, J Chen, S Carlson, H Cheng, C Silvin, X Yang, C Van Waes, Z Chen Death agonist antibody against TRAILR2/DR5/TNFRSF10B enhances birinapant anti-tumor activity in HPV-positive head and neck squamous cell carcinomas Scientific Reports, 2021-03-18;11(1):6392. 2021-03-18 [PMID: 33737574] (Western Blot, Human) Western Blot Human
Roy Xiao, Yi An, Wenda Ye, Adeeb Derakhshan, Hui Cheng, Xinping Yang et al. Dual Antagonist of cIAP/XIAP ASTX660 Sensitizes HPV(−) and HPV(+) Head and Neck Cancers To TNF alpha , TRAIL, and Radiation Therapy Clinical Cancer Research 2019-11-01 [PMID: 31266830]
S Lee, JY Lee, EW Lee, S Park, DH Kang, C Min, DJ Lee, D Kang, J Song, J Kwon, SW Kang Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-?-Induced Apoptosis via Different Routes Cell Rep, 2019-02-19;26(8):2194-2211.e6. 2019-02-19 [PMID: 30784599] (Western Blot, Human) Western Blot Human
GA Ward, EJ Lewis, JS Ahn, CN Johnson, JF Lyons, V Martins, JM Munck, SJ Rich, T Smyth, NT Thompson, PA Williams, NE Wilsher, NG Wallis, G Chessari ASTX660, a novel non-peptidomimetic antagonist of cIAP1/2 and XIAP, potently induces TNF-? dependent apoptosis in cancer cell lines and inhibits tumor growth Mol. Cancer Ther., 2018-04-25;0(0):. 2018-04-25 [PMID: 29695633] (Western Blot, Human) Western Blot Human
Xuefeng Deng, Wei Zou, Min Xiong, Zekun Wang, John F. Engelhardt, Shui Qing Ye et al. Human Parvovirus Infection of Human Airway Epithelia Induces Pyroptotic Cell Death by Inhibiting Apoptosis Journal of Virology 2017-12-15 [PMID: 29021400]
V Liapis, A Zysk, M DeNichilo, I Zinonos, S Hay, V Panagopoul, A Shoubridge, C Difelice, V Ponomarev, W Ingman, GJ Atkins, DM Findlay, ACW Zannettino, A Evdokiou Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma Cancer Med, 2017-08-10;0(0):. 2017-08-10 [PMID: 28799237] (Western Blot, Human) Western Blot Human
Tchoghandjian A, Souberan A, Tabouret E et al. Inhibitor of apoptosis protein expression in glioblastomas and their in vitro and in vivo targeting by SMAC mimetic GDC-0152 Cell Death Dis 8/5/2016 [PMID: 27490930]
Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies Molecules, 2016-07-30;21(8):. 2016-07-30 [PMID: 27483232] (Flow Cytometry, Human) Flow Cytometry Human
Subrahmanya D. Vallabhapurapu, Sunil K. Noothi, Derek A. Pullum, Charles H. Lawrie, Rachel Pallapati, Veena Potluri et al. Transcriptional repression by the HDAC4–RelB–p52 complex regulates multiple myeloma survival and growth Nature Communications 2015-10-12 [PMID: 26455434]
Lee E, Seong D, Seo J, Jeong M, Lee H, Song J USP11-dependent selective cIAP2 deubiquitylation and stabilization determine sensitivity to Smac mimetics. Cell Death Differ, 2015-01-23;22(9):1463-76. 2015-01-23 [PMID: 25613375] (Western Blot, Human) Western Blot Human
Show All 17 Publications.

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Additional cIAP-2/HIAP-1 Products

Blogs on cIAP-2/HIAP-1.

Check out the latest blog posts on cIAP-2/HIAP-1.
cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and ...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol BIRC3
Uniprot