![Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-2120249403823.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, DrSpecies Glossary |
| Applications | WB, IHC |
| Clone | HL1268 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Alkaline Phosphatase, Intestinal. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ALPI |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Alkaline Phosphatase, Intestinal Antibody (NBP3-25355)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ALPI |
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Alkaline phosphatase (intestinal) antibody [HL1268] detects secreted Alkaline phosphatase (intestinal) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Alkaline phosphatase (intestinal) stained by Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-2120249374927.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Alkaline phosphatase (intestinal) antibody [HL1268] detects secreted Alkaline phosphatase (intestinal) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Alkaline phosphatase (intestinal) stained by Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-2120249351323.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-2120249393597.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-212024940823.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-2120249403890.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Alkaline phosphatase (intestinal) antibody [HL1268] detects secreted Alkaline phosphatase (intestinal) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Alkaline phosphatase (intestinal) stained by Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25355_rabbit-alkaline-phosphatase-intestinal-mab-hl1268-azide-bsa-free-212024940846.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase, Intestinal Antibody (HL1268) - Azide and BSA Free [NBP3-25355] - Alkaline phosphatase (intestinal) antibody [HL1268] detects secreted Alkaline phosphatase (intestinal) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Alkaline phosphatase (intestinal) stained by Alkaline phosphatase (intestinal) antibody [HL1268] (NBP3-25355) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")