![Western Blot: CHD1 Antibody [NB100-60411] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CHD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human CHD1 Polyclonal Antibody (Catalog # NB100-60411) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CHD1 at approximately 260 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/CHD1-Antibody-Knockout-Validated-NB100-60411-img0007.jpg "Western Blot: CHD1 Antibody [NB100-60411] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CHD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human CHD1 Polyclonal Antibody (Catalog # NB100-60411) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CHD1 at approximately 260 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
| Reactivity | Hu, Mu, ChSpecies Glossary |
| Applications | WB, IP, ChIP, KD, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1660 and 1710 of human chromodomain helicase DNA binding protein 1 using the numbering given in entry NP_001261.2 (GeneID 1105). |
| Predicted Species | Chicken (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CHD1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB in human was reported in the following publication: PMID: 21880597 |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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![Western Blot: CHD1 Antibody [NB100-60411] - Detection of Human CHD1 on HeLa whole cell lysate using NB100-60411. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody NB100-60410.](http://images.novusbio.com/fullsize/CHD1-Antibody-Western-Blot-NB100-60411-img0005.jpg "Western Blot: CHD1 Antibody [NB100-60411] - Detection of Human CHD1 on HeLa whole cell lysate using NB100-60411. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody NB100-60410.")
![Chromatin Immunoprecipitation Sequencing: CHD1 Antibody [NB100-60411] - Localization of CHD1 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CHD1 antibody NB100-60411 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CHD1 binding within a 3.5 Mb region of the human X chromosome as detected using anti-CHD1 antibody NB100-60411. ChIP-seq validation performed by Diogenode, Denville, NJ.](http://images.novusbio.com/fullsize/CHD1-Antibody-Chromatin-Immunoprecipitation-Sequencing-NB100-60411-img0006.jpg "Chromatin Immunoprecipitation Sequencing: CHD1 Antibody [NB100-60411] - Localization of CHD1 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CHD1 antibody NB100-60411 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CHD1 binding within a 3.5 Mb region of the human X chromosome as detected using anti-CHD1 antibody NB100-60411. ChIP-seq validation performed by Diogenode, Denville, NJ.")
![Chromatin Immunoprecipitation: CHD1 Antibody [NB100-60411] - ChIP-chip scatter plot of anti-CHD1 (NB100-60411) enriched DNA binding sites versus input reference DNA. CHD1 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-CHD1 ChIP, normal rabbit IgG showed little enrichment.](http://images.novusbio.com/fullsize/CHD1-Antibody-Chromatin-Immunoprecipitation-NB100-60411-img0003.jpg "Chromatin Immunoprecipitation: CHD1 Antibody [NB100-60411] - ChIP-chip scatter plot of anti-CHD1 (NB100-60411) enriched DNA binding sites versus input reference DNA. CHD1 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-CHD1 ChIP, normal rabbit IgG showed little enrichment.")
![Immunocytochemistry/ Immunofluorescence: CHD1 Antibody [NB100-60411] - Chromatin marks associated with transcriptional elongation are increased in the absence of Lsh. Western analysis for detection of Chd1 protein comparing whole cell extracts derived from Lsh-/-MEFs targeted by Chd1 siRNA or control siRNA. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0009163), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CHD1-Antibody-Knockdown-Validated-NB100-60411-img0008.jpg "Immunocytochemistry/ Immunofluorescence: CHD1 Antibody [NB100-60411] - Chromatin marks associated with transcriptional elongation are increased in the absence of Lsh. Western analysis for detection of Chd1 protein comparing whole cell extracts derived from Lsh-/-MEFs targeted by Chd1 siRNA or control siRNA. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0009163), licensed under a CC-BY license.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")