| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6195-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02966-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6195-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14478-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98902-50ul | |||
![Western Blot: CHD1 Antibody [NB100-60411] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CHD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human CHD1 Polyclonal Antibody (Catalog # NB100-60411) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CHD1 at approximately 260 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/CHD1-Antibody-Knockout-Validated-NB100-60411-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CHD1 Antibody [NB100-60411] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CHD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human CHD1 Polyclonal Antibody (Catalog # NB100-60411) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CHD1 at approximately 260 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: CHD1 Antibody [NB100-60411] - Detection of Human CHD1 on HeLa whole cell lysate using NB100-60411. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody NB100-60410.](http://images.novusbio.com/fullsize/CHD1-Antibody-Western-Blot-NB100-60411-img0005.jpg)
![Chromatin Immunoprecipitation Sequencing: CHD1 Antibody [NB100-60411] - Localization of CHD1 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CHD1 antibody NB100-60411 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CHD1 binding within a 3.5 Mb region of the human X chromosome as detected using anti-CHD1 antibody NB100-60411. ChIP-seq validation performed by Diogenode, Denville, NJ.](http://images.novusbio.com/fullsize/CHD1-Antibody-Chromatin-Immunoprecipitation-Sequencing-NB100-60411-img0006.jpg)
![Chromatin Immunoprecipitation: CHD1 Antibody [NB100-60411] - ChIP-chip scatter plot of anti-CHD1 (NB100-60411) enriched DNA binding sites versus input reference DNA. CHD1 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-CHD1 ChIP, normal rabbit IgG showed little enrichment.](http://images.novusbio.com/fullsize/CHD1-Antibody-Chromatin-Immunoprecipitation-NB100-60411-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: CHD1 Antibody [NB100-60411] - Chromatin marks associated with transcriptional elongation are increased in the absence of Lsh. Western analysis for detection of Chd1 protein comparing whole cell extracts derived from Lsh-/-MEFs targeted by Chd1 siRNA or control siRNA. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0009163), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CHD1-Antibody-Knockdown-Validated-NB100-60411-img0008.jpg)
![Western Blot: CHD1 Antibody [NBP3-02966] - Analysis of extracts of Jurkat cells, using CHD1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/CHD1-Antibody-Western-Blot-NBP3-02966-img0006.jpg "Western Blot: CHD1 Antibody [NBP3-02966] - Analysis of extracts of Jurkat cells, using CHD1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP3-02966] - Rat brain using CHD1 antibody (NBP3-02966) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP3-02966-img0003.jpg)
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP3-02966] - Mouse brain using CHD1 antibody (NBP3-02966) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP3-02966-img0001.jpg)
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP3-02966] - Human liver damage using CHD1 antibody (NBP3-02966) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP3-02966-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CHD1 Antibody [NBP3-02966] - Western blot analysis of extracts from normal (control) and CHD1 knockout (KO) 293T cells, using CHD1 antibody (NBP3-02966) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/CHD1-Antibody-Knockout-Validated-NBP3-02966-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CHD1 Antibody [NBP2-14478] - Staining of human cell line RH-30 shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14478-img0003.jpg "Immunocytochemistry/Immunofluorescence: CHD1 Antibody [NBP2-14478] - Staining of human cell line RH-30 shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining in human testis and skeletal muscle tissues.. Corresponding CHD1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-14478-img0010.jpg)
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human placenta shows moderate to strong nuclear positivity.](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-14478-img0005.jpg)
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human endometrium shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-14478-img0006.jpg)
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-14478-img0007.jpg)
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-14478] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-14478-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CHD1 Antibody (1G2) [H00001105-M04] - Analysis of monoclonal antibody to CHD1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/CHD1-Antibody-1G2-Immunocytochemistry-Immunofluorescence-H00001105-M04-img0001.jpg "Immunocytochemistry/Immunofluorescence: CHD1 Antibody (1G2) [H00001105-M04] - Analysis of monoclonal antibody to CHD1 on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: CHD1 Antibody (1G2) [H00001105-M04] - Detection limit for recombinant GST tagged CHD1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CHD1-Antibody-1G2-Sandwich-ELISA-H00001105-M04-img0002.jpg)
![Immunoprecipitation: CHD1 Antibody [NB100-60410] - Detection of Human CHD1 on HeLa whole cell lysate using NB100-60410. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody NB100-60411.](http://images.novusbio.com/fullsize/CHD1-Antibody-Western-Blot-NB100-60410-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD1 Antibody [NB100-60410] - Detection of Human CHD1 on HeLa whole cell lysate using NB100-60410. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody NB100-60411.")
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-98902] - Mouse kidney with rabbit polyclonal antibody (1:300).](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-98902-img0002.jpg "Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-98902] - Mouse kidney with rabbit polyclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: CHD1 Antibody [NBP2-98902] - Human kidney with rabbit polyclonal antibody (1:300).](http://images.novusbio.com/fullsize/CHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-98902-img0001.jpg)
![Western Blot: CHD1 Antibody [NBP3-18661] - Figure 1. Western blot analysis using the antibody directed against CHD1 Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 ug) with the antibody against CHD1 diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/CHD1 Antibody-Western Blot-NBP3-18661-img0001.jpg "Western Blot: CHD1 Antibody [NBP3-18661] - Figure 1. Western blot analysis using the antibody directed against CHD1 Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 ug) with the antibody against CHD1 diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.")