![Immunocytochemistry/Immunofluorescence: CEP290 Antibody [NBP1-52132] - Overnight staining with antibody at 2.5 ug/mL of cell lines OPCT-1 (A) and MDA468 (B) with Alexa Fluor 568 (red) and nuclear counter staining with DAPI (blue), provided by Dr S, McArdle, and obtained by Sumanjeet Malhi, John van Geest Research Centre, Nottingham, UK.](http://images.novusbio.com/fullsize/CEP290-Antibody-Immunofluorescence-NBP1-52132-img0001.jpg "Immunocytochemistry/Immunofluorescence: CEP290 Antibody [NBP1-52132] - Overnight staining with antibody at 2.5 ug/mL of cell lines OPCT-1 (A) and MDA468 (B) with Alexa Fluor 568 (red) and nuclear counter staining with DAPI (blue), provided by Dr S, McArdle, and obtained by Sumanjeet Malhi, John van Geest Research Centre, Nottingham, UK.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, ICC/IF, IHC, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-RNSKHLKQQQYRAEN corresponding to internal region according to NP_079390.3. |
| Epitope | C-RNSKHLKQQQYRAEN |
| Specificity | Despite similarity of the immunizing peptide with the mouse protein, this antibody was demonstrated to weak for mouse Cep290. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | CEP290 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ICC/IF: Formaldehyde-fixed cell lines derived from prostate cancer and from breast cancer show typical centrosomal staining. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CEP290 Antibody (NBP1-52132)Find related products by research area.
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| Gene Symbol | CEP290 |
| Entrez | |
| Uniprot |
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![Immunohistochemistry-Paraffin: CEP290 Antibody [NBP1-52132] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.](http://images.novusbio.com/fullsize/CEP290-Antibody-Immunohistochemistry-Paraffin-NBP1-52132-img0004.jpg "Immunohistochemistry-Paraffin: CEP290 Antibody [NBP1-52132] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.")
![Immunohistochemistry-Paraffin: CEP290 Antibody [NBP1-52132] - Staining of paraffin embedded Human Prostate with antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/CEP290-Antibody-Immunohistochemistry-Paraffin-NBP1-52132-img0003.jpg "Immunohistochemistry-Paraffin: CEP290 Antibody [NBP1-52132] - Staining of paraffin embedded Human Prostate with antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.")
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