![Western Blot: Meckelin Antibody [NBP1-06590] - Immunodetection of Meckelin (120kDa) in whole cell extracts of HEK293 cells.](http://images.novusbio.com/fullsize/Meckelin-Antibody-Western-Blot-NBP1-06590-img0003.jpg "Western Blot: Meckelin Antibody [NBP1-06590] - Immunodetection of Meckelin (120kDa) in whole cell extracts of HEK293 cells.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Synthetic peptide derived from Cter domain of human Meckelin protein |
| Specificity | Reacts specifically with human 111 kDa protein |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TMEM67 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-Frozen, ICC/IF applications reported in scientific literature (PMID: 23516626) |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Antiserum |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Unpurified |
| Reconstitution Instructions | Lyophilized powder, reconstituted in distilled water. |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry: Meckelin Antibody [NBP1-06590] - Confocal maximum projection of acetylated alpha-tubulin (red) and meckelin (green) labelling in sections from unaffected and affected kidneys. In unaffected kidneys, meckelin was present within the cytoplasm of the epithelial cells and strongly localised to the base of the cilium. In contrast, meckelin was distributed along the length of the ciliary axonemes of affected cyst-lining cells. Meckelin distribution within the cytoplasm of cyst lining cells was similar to observations in unaffected epithelia. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28487520/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Meckelin-Antibody-Immunohistochemistry-NBP1-06590-img0004.jpg "Immunohistochemistry: Meckelin Antibody [NBP1-06590] - Confocal maximum projection of acetylated alpha-tubulin (red) and meckelin (green) labelling in sections from unaffected and affected kidneys. In unaffected kidneys, meckelin was present within the cytoplasm of the epithelial cells and strongly localised to the base of the cilium. In contrast, meckelin was distributed along the length of the ciliary axonemes of affected cyst-lining cells. Meckelin distribution within the cytoplasm of cyst lining cells was similar to observations in unaffected epithelia. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28487520/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Meckelin Antibody [NBP1-06590] - Staining of kidney tissue section with Meckelin Antibody (NBP1-06590).](http://images.novusbio.com/fullsize/Meckelin-Antibody-Immunohistochemistry-Paraffin-NBP1-06590-img0002.jpg "Immunohistochemistry-Paraffin: Meckelin Antibody [NBP1-06590] - Staining of kidney tissue section with Meckelin Antibody (NBP1-06590).")
![Immunocytochemistry/ Immunofluorescence: Meckelin Antibody [NBP1-06590] - Meckelin expression in unaffected & affected lamb kidney epithelial cells & primary cilia. (a, left) Immunoblot showing expression of meckelin (~100 KDa) in cultured epithelial cells from an unaffected lamb kidney, & an affected lamb kidney. beta -actin indicates equivalent protein loading in each lane. (a, right) Quantification of the meckelin band in the immunoblot, normalised to the loading control, shows no significant difference in relative protein expression. (b) Confocal maximum projection of acetylated alpha -tubulin (red) & meckelin (green) labelling in sections from unaffected & affected kidneys. In unaffected kidneys, meckelin was present within the cytoplasm of the epithelial cells & strongly localised to the base of the cilium. In contrast, meckelin was distributed along the length of the ciliary axonemes of affected cyst-lining cells. Meckelin distribution within the cytoplasm of cyst lining cells was similar to observations in unaffected epithelia (c) Acetylated alpha -tubulin (red) & meckelin (green) labelling on putative ciliary fragments located in the cyst lumen of affected kidneys (yellow indicates co-localisation in the merged image). Numerous smaller particles contained in the cyst luminal contents were also labeled with meckelin, but were not labeled with acetylated alpha -tubulin. Scale bars = 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28487520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06590_rabbit-polyclonal-meckelin-antibody-310202416163791.jpg "Immunocytochemistry/ Immunofluorescence: Meckelin Antibody [NBP1-06590] - Meckelin expression in unaffected & affected lamb kidney epithelial cells & primary cilia. (a, left) Immunoblot showing expression of meckelin (~100 KDa) in cultured epithelial cells from an unaffected lamb kidney, & an affected lamb kidney. beta -actin indicates equivalent protein loading in each lane. (a, right) Quantification of the meckelin band in the immunoblot, normalised to the loading control, shows no significant difference in relative protein expression. (b) Confocal maximum projection of acetylated alpha -tubulin (red) & meckelin (green) labelling in sections from unaffected & affected kidneys. In unaffected kidneys, meckelin was present within the cytoplasm of the epithelial cells & strongly localised to the base of the cilium. In contrast, meckelin was distributed along the length of the ciliary axonemes of affected cyst-lining cells. Meckelin distribution within the cytoplasm of cyst lining cells was similar to observations in unaffected epithelia (c) Acetylated alpha -tubulin (red) & meckelin (green) labelling on putative ciliary fragments located in the cyst lumen of affected kidneys (yellow indicates co-localisation in the merged image). Numerous smaller particles contained in the cyst luminal contents were also labeled with meckelin, but were not labeled with acetylated alpha -tubulin. Scale bars = 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28487520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Bioactivity Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images2/Sonic_Hedgehog_464SH_2840.jpg)
![SDS-PAGE Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images2/Sonic_Hedgehog_464-SH_341.jpg)
![SEC-MALS Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images/464-sh_recombinant-mouse-sonic-hedgehog-shh-c25ii-n-terminus-sec-mals-642023224324.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")