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IQCB1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: IQCB1 Antibody [NBP2-14126] - Staining of human cell line U-2 OS shows localization to nucleoplasm, microtubules, cytokinetic bridge & mitotic spindle.
Immunohistochemistry-Paraffin: IQCB1 Antibody [NBP2-14126] - Staining of human retina shows strong cytoplasmic positivity in cones.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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IQCB1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: AVTLQRAALKFLAKCRKKKKLFAPWRGLQELTDARRVELKKRVDDYVRRHLGSPMSDVVSRELHAQAQERLQHYFMGRALEERAQQHREALIAQISTNVEQLMK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IQCB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
IQCB1 Protein (NBP2-14126PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IQCB1 Antibody

  • IQ calmodulin-binding motif containing 1
  • IQ motif containing B1
  • KIAA0036nephrocystin 5
  • Nephrocystin-5
  • NPHP5IQ calmodulin-binding motif-containing protein 1
  • p53 and DNA damage-regulated IQ motif protein
  • PIQ
  • SLSN5

Background

CEP290 activates ATF4 mediated transcription and is required for the correct localization of ciliary and phototransduction proteins in retinal photoreceptor cells; may play a role in ciliary transport processes. CEP290 is ubiquitously expressed; strongly in placenta and weakly in brain. There are two named isoforms. Defects in CEP290 are a cause of Joubert syndrome type 5 (JBTS5) [MIM:610188], Senior-Loken syndrome type 6 (SLSN6) [MIM:610189], Leber congenital amaurosis type 10 (LCA10) [MIM:611755] and Meckel syndrome type 4 (MKS4) [MIM:611134]. Antibodies against CEP290 are present in sera from patients with cutaneous T cell lymphomas, but not in the healthy control population.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol IQCB1