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CEACAM8/CD66b Antibody (913542) [Unconjugated]

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Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) and human peripheral blood granulocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CEACAM-8/CD66b Monoclonal Antibody (Catalog # MAB4246) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CEACAM-8/CD66b at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) and human peripheral blood granulocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CEACAM-8/CD66b Monoclonal Antibody ...read more
Human peripheral blood granulocytes were stained with Mouse Anti-Human CEACAM‑8/CD66b Monoclonal Antibody (Catalog # MAB4246, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed ...read more
CEACAM‑8/CD66b was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CEACAM‑8/CD66b Monoclonal Antibody (Catalog # MAB4246) at 0.5 µg/ml for 1 hour at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
913542
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB4246U-100UG
Alexa Fluor 405
FAB4246V-100UG
Alexa Fluor 488
FAB4246G-100UG
Alexa Fluor 532
FAB4246X
Alexa Fluor 594
FAB4246T-100UG
Alexa Fluor 647
FAB4246R-100UG
Alexa Fluor 700
FAB4246N-100UG
Alexa Fluor 750
FAB4246S-100UG
Allophycocyanin/Cy7
FAB4246APCCY7
Biotin
FAB4246B-100
CoraFluor 1
FAB4246CL1
DyLight 350
FAB4246D
DyLight 405
FAB4246E
DyLight 488
FAB4246K
DyLight 550
FAB4246L
DyLight 594
FAB4246M
DyLight 650
FAB4246W
DyLight 680
FAB4246Y
DyLight 755
FAB4246Z
FITC
FAB4246F
HRP
FAB4246H
Janelia Fluor 525
FAB4246JF525
Janelia Fluor 549
FAB4246I
Janelia Fluor 646
FAB4246J
Janelia Fluor 669
FAB4246JF669
PE/Atto594
FAB4246PEATT594
PE/Cy5.5
FAB4246PECY55
PE/Cy7
FAB4246PECY7
PerCP
FAB4246C
mFluor Violet 450 SE
FAB4246MFV450
mFluor Violet 500 SE
FAB4246MFV500
mFluor Violet 610 SE
FAB4246MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4246-SP
Carrier Free
FAB4246B-100
Carrier Free
FAB4246V-100UG
Carrier Free
FAB4246U-100UG
Carrier Free
FAB4246T-100UG
Carrier Free
FAB4246S-100UG
Carrier Free
FAB4246R-100UG
Carrier Free
FAB4246N-100UG
Carrier Free
FAB4246G-100UG
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CEACAM8/CD66b Antibody (913542) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
E.coli-derived recombinant human CEACAM-8/CD66b
Gln35-His141
Accession # P31997
Specificity
Detects human CEACAM-8/CD66b in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CEACAM8
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 0.5-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using
MAB4246 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM8/CD66b Antibody (913542) [Unconjugated]

  • Carcinoembryonic antigen CGM6
  • carcinoembryonic antigen gene family member 6
  • carcinoembryonic antigen-related cell adhesion molecule 8
  • CD66b antigen
  • CD66b
  • CD67 antigen
  • CD67
  • CEACAM8
  • CEACAM-8
  • CGM6
  • NCA-95
  • Non-specific cross-reacting antigen NCA-95

Background

CEACAM-8 (Carcinoembryonic Antigen-related Cell Adhesion Molecule 8), also known as CD66b, CD67 and NCA-95, is a 90 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and eosinophils, and serves as a binding partner for CEACAM-6 and Galectin-3. Mature human
CEACAM-8 is a 287 amino acid GPI-linked glycoprotein. It contains one V-type and two C2-type Ig-like domains. No definitive rodent CEACAM-8 has been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CEACAM8/CD66b Antibody (MAB4246)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CEACAM8
Uniprot