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CDC25A Antibody

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Immunocytochemistry/ Immunofluorescence: CDC25A Antibody [NBP3-17816] - Staining of human cell line HAP1 shows localization to nucleoplasm.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CDC25A Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TATLSDEDDGFVDLLDGENLKNEEETPSCMASLWTAPLVMRTTNLDNRCKLFDSPSLCSSSTRSVLKRPERSQEESPPGSTKRRKSMSGASPKESTNPEKAHETLHQSLSLASSPKGTIENILDNDPRDL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDC25A
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for CDC25A Antibody

  • CDC25A
  • CDC25A2
  • CDC25A2-CAG isoform
  • cell division cycle 25 homolog A (S. cerevisiae)
  • cell division cycle 25 homolog A (S. pombe)
  • cell division cycle 25A
  • Dual specificity phosphatase Cdc25A
  • EC 3.1.3.48
  • M-phase inducer phosphatase 1

Background

The activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S phase and G2 for CDC25B. CDC25C is mainly expressed in G2 phase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CDC25A