CD99 Antibody (MEM-131) Summary
Additional Information |
Clone MEM-131 was used by HLDA to establish CD designation. |
Immunogen |
HPB-ALL human peripheral blood leukemia T-cell line |
Specificity |
The antibody MEM-131 reacts with CD99R, an epitope restricted to a subset of CD99 molecule expressed on myeloid cells, NK cells and T lymphocytes. HLDA V; WS Code AS S020 HLDA V; WS Code T T-E2.02 HLDA V; WS Code T T-017 |
Isotype |
IgM |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
CD99 |
Purity |
Multi-step |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 1 ug/ml
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Paraffin 10 ug/ml
- Immunoprecipitation 1:50
- Western Blot 1:1000
|
Application Notes |
Western Blot: Use under non-reducing conditions. Antibody purity is > 95% (by SDS-PAGE). |
Reactivity Notes
Cross-reacts with Human. Not yet tested in other species.
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze. |
Buffer |
TBS (pH 8.0) |
Preservative |
0.09% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Multi-step |
Alternate Names for CD99 Antibody (MEM-131)
Background
CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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