CD90/Thy1 Antibody (OX-7) - Azide and BSA Free Summary
Immunogen |
Rat Thy 1 antigen |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
THY1 |
Purity |
Protein A purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- CyTOF-ready
- Flow Cytometry 1:50-1:100
- Immunocytochemistry/ Immunofluorescence 1:10-1:500
- Immunohistochemistry
- Immunohistochemistry-Frozen 1:10-1:500
- Immunoprecipitation 1:10-1:500
- Western Blot 1:100-1:2000
|
Application Notes |
For Flow Cytometry: Use 10 uL of the suggested working dilution to label 10^6 cells in 100 uL. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
Protein A purified |
Alternate Names for CD90/Thy1 Antibody (OX-7) - Azide and BSA Free
Background
CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1. Thy1.1, expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues. Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab' of MRC OX-7 for rat Thy1 is 3 x 10^9m^1 and for mouse Thy1.1 is 3 x 10^8m-^1(1). Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (5).This clone has been reported to induce glomerular nephritis in Wistar rats (8).This product is routinely tested in flow cytometry on rat thymocytes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Video Protocols
FAQs for CD90/Thy1 Antibody (NBP2-80661). (Showing 1 - 1 of 1 FAQ).
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We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
- I would recommend one of theses <a href="http://www.novusbio.com/productsearch/CD90%252FThy1#fq=common_name%3A%22CD90%2FThy1%22&fq=species%3A%22Canine%22" target="_self">CD90 antibodies</a>, as these have all been validated for use with dog/canine samples.