Novus Biologicals products are now on bio-techne.com

CD9 Antibody (5G6) - BSA Free

Images

 
Western Blot: CD9 Antibody (5G6) [NBP2-22187] - Analysis using CD9 mAb against HEK293 (1) and CD9(AA: 37-228)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: CD9 Antibody (5G6) [NBP2-22187] - Human endometrial stromal cells stained with anti-CD9 antibody and detected with anti-mouse IgG AlexaFluor 488 secondary antibody (Green). ...read more
Immunohistochemistry-Paraffin: CD9 Antibody (5G6) [NBP2-22187] - Analysis of kidney cancer tissues using CD9 mouse mAb with DAB staining.
Flow Cytometry: CD9 Antibody (5G6) [NBP2-22187] - Analysis of Jurkat cells using CD9 mouse mAb (green) and negative control (red).
Immunohistochemistry-Paraffin: CD9 Antibody (5G6) [NBP2-22187] - Analysis of cervical cancer tissues using CD9 mouse mAb with DAB staining.
ELISA: CD9 Antibody (5G6) [NBP2-22187] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
5G6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

CD9 Antibody (5G6) - BSA Free Summary

Immunogen
Synthesized peptide of human CD9.
Localization
Cytoplasm, Membrane
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD9
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence Validated from a verified customer review
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-22187 in the following applications:

Publications
Read Publications using
NBP2-22187 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for CD9 Antibody (5G6) - BSA Free

  • BA2
  • BA-2/p24 antigen
  • BTCC-1
  • CD9 antigen
  • CD9 molecule
  • CD9
  • Cell growth-inhibiting gene 2 protein
  • DRAP-27
  • Leukocyte antigen MIC3
  • MIC3
  • motility related protein-1,5H9 antigen
  • Motility-related protein
  • MRP-1FLJ99568
  • p24
  • tetraspanin-29
  • TSPAN29
  • TSPAN-29
  • TSPAN29MIC3CD9 antigen (p24)

Background

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

7268-CT
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NB100-65805
Species: Gt, Hu, Mu, Pm, Sh
Applications: CyTOF-ready, DB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-42225
Species: Ca, Hu
Applications: B/N, DB, EM, ELISA, Flow-IC, Flow, Func, ICC/IF, IHC-WhMt, IP, In vitro, WB
NBP2-21792
Species: Hu
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, WB
NB400-156
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Dual ISH-IHC, ELISA, Flow, ICC/IF, IHC, IP, Simple Western, WB
MAB17781
Species: Hu
Applications: Block, CyTOF-ready, Flow, ICC, IP, KO
MAB4609
Species: Mu
Applications: CyTOF-ready, Flow, ICC
AF1126
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, IP, WB
BBA10
Species: Hu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
236-EG
Species: Hu
Applications: BA
AF4117
Species: Rt
Applications: IHC, WB
NBP1-91258
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP2-25196
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC/IF, In vitro, WB
NBP2-22124
Species: Hu, Mu, Pm
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NB500-393
Species: Hu
Applications: CyTOF-ready, Flow, IHC, IHC-Fr, IP, WB
MAB7616
Species: Hu
Applications: CyTOF-ready, Flow, WB
AF796
Species: Mu
Applications: AdBlk, IHC, WB

Publications for CD9 Antibody (NBP2-22187)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IA, WB, Western Blot.


Filter By Application
IA
(1)
WB
(3)
Western Blot
(1)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP2-22187 Applications Species
Sheng-Mou Hou, Chih-Yang Lin, Yi-Chin Fong, Chih-Hsin Tang Hypoxia-regulated exosomes mediate M2 macrophage polarization and promote metastasis in chondrosarcoma Aging (Albany NY) 2023-11-30 [PMID: 37993261]
Kolka CM, Webster J, Lepletier A et al. C5b-9 Membrane Attack Complex Formation and Extracellular Vesicle Shedding in Barrett's Esophagus and Esophageal Adenocarcinoma Frontiers in immunology 2022-03-08 [PMID: 35345676] (IA, Human) IA Human
Zar� M, Campodonico J, Cosentino N et al. Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest International Journal of Molecular Sciences 2021-07-28 [PMID: 34360827] (Western Blot) Western Blot
Choi H, Kim Y, Mirzaaghasi A et al. Exosome-based delivery of super-repressor I kappa B alpha relieves sepsis-associated organ damage and mortality Sci Adv 2020-04-01 [PMID: 32285005] (WB, Human) WB Human
Ren W, Hou J, Yang C et al. Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery J. Exp. Clin. Cancer Res. 2019-02-08 [PMID: 30736829] (WB, Human) WB Human
Su C, Lai Y, Bamodu O et al. Cancer-Associated Fibroblasts Release Exosomal CBFBThat Dictate an Aggressive Bone Metastasis Phenotype in Breast Cancer Research Square 2020-12-29 (WB, Human) WB Human
Chhoy P, Brown C, Amante J, Mercurio A Protocol for the separation of extracellular vesicles by ultracentrifugation from in vitro cell culture models STAR Protocols 2021-03-01 [PMID: 33554138]

Review for CD9 Antibody (NBP2-22187) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-22187:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence CD9 NBP2-22187
Enlarge
5
reviewed by:
Gareth Healey
IF Human 01/18/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedHuman endometrial stromal cells
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD9 Antibody (NBP2-22187) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CD9 Products

Research Areas for CD9 Antibody (NBP2-22187)

Find related products by research area.

Blogs on CD9.

Glypican 3 as a biomarker for gastro-esophageal adenocarcinoma
By Jamshed Arslan, Pharm. D., PhD. Gastroesophageal adenocarcinoma originates from the glandular epithelium of the esophagus, gastroesophageal junction and stomach. The incidence of gastroesophageal adenocarcinoma is ...  Read full blog post.

Tools for Isolation, Quantification and Analysis of Exosomes
Exosomes are spherical to cup-shaped bilayered membrane enclosed nanosize vesicles (30-100 nm) which have the ability to shuttle active cargoes between cells. Johnstone et al. 1987 pioneered in documenting the generation of exosomes in differentiat...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Gareth Healey
01/18/2016
Application: IF
Species: Human

Bioinformatics

Gene Symbol CD9