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CD53 Antibody (SR1376)

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Western Blot: CD53 Antibody (SR1376) [NBP3-21921] - Western blot analysis using NBP3-21921 in Jurkat cell lysate.
Western Blot: CD53 Antibody (SR1376) [NBP3-21921] - Western blot analysis using NBP3-21921 in Jurkat cell lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
SR1376
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CD53 Antibody (SR1376) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human CD53 (Uniprot #: P19397)
Specificity
Detects endogenous levels of total CD53
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CD53
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for CD53 Antibody (SR1376)

  • antigen MOX44 identified by monoclonal MRC-OX44
  • CD53 antigentetraspanin-25
  • CD53 glycoprotein
  • CD53 molecule
  • CD53 tetraspan antigen
  • CD53
  • cell surface antigen
  • Cell surface glycoprotein CD53
  • MOX44
  • MOX44transmembrane glycoprotein
  • Tetraspanin-25
  • TSPAN25
  • tspan-25
  • TSPAN25leukocyte surface antigen CD53

Background

The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD53 Antibody (NBP3-21921) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD53