| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB4624A | |||
| Carrier Free | MAB4624-SP | |||
| Carrier Free | FAB4624V-100UG | |||
| Carrier Free | FAB4624U-100UG | |||
| Carrier Free | FAB4624T-100UG | |||
| Carrier Free | FAB4624S-100UG | |||
| Carrier Free | FAB4624R-100UG | |||
| Carrier Free | FAB4624N-100UG | |||
| Carrier Free | FAB4624G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12059-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47859-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12058-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11477 | |||
![Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) PE.](http://images.novusbio.com/fullsize/CD53-Antibody-MEM-53-Flow-Cytometry-NB500-393-img0002.jpg "Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) PE.")
![Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD53-Antibody-MEM-53-Flow-Cytometry-NB500-393-img0001.jpg)
![Western Blot: CD53 Antibody (HD77) - Chimeric [NBP3-12059] - Human lymph node (A), human thymus (B) and human spleen (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HD77 (NBP3-12059) at 0.1 ug/ml, 0.3 ug/ml and 0.5 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD53-Antibody-HD77-Chimeric-Western-Blot-NBP3-12059-img0001.jpg "Western Blot: CD53 Antibody (HD77) - Chimeric [NBP3-12059] - Human lymph node (A), human thymus (B) and human spleen (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HD77 (NBP3-12059) at 0.1 ug/ml, 0.3 ug/ml and 0.5 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CD53 Antibody (HD77) - Chimeric [NBP3-12059] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version HD77 (NBP3-12059) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12059, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD53-Antibody-HD77-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12059-img0002.jpg)
![Western Blot: CD53 Antibody (JE60-38) [NBP3-32159] - Western blot analysis of CD53 on different lysates with Rabbit anti-CD53 antibody (NBP3-32159) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Raji cell lysate Lane 3: Ramos cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 35-42 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32159) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32159_rabbit-cd53-mab-je60-38-278202416331213.jpg "Western Blot: CD53 Antibody (JE60-38) [NBP3-32159] - Western blot analysis of CD53 on different lysates with Rabbit anti-CD53 antibody (NBP3-32159) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Raji cell lysate Lane 3: Ramos cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 35-42 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32159) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD53 Antibody (JE60-38) [NBP3-32159] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD53 antibody (NBP3-32159) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32159) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32159_rabbit-cd53-mab-je60-38-298202414333654.jpg)
![Immunocytochemistry/ Immunofluorescence: CD53 Antibody (JE60-38) [NBP3-32159] - Immunocytochemistry analysis of Jurkat cells labeling CD53 with Rabbit anti-CD53 antibody (NBP3-32159) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD53 antibody (NBP3-32159) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32159_rabbit-cd53-mab-je60-38-29202410334023.jpg)
![Western Blot: CD53 Antibody (SR1376) [NBP3-21921] - Western blot analysis using NBP3-21921 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-21921_rabbit-cd53-mab-sr1376-6720239533737.jpg "Western Blot: CD53 Antibody (SR1376) [NBP3-21921] - Western blot analysis using NBP3-21921 in Jurkat cell lysate.")
![Western Blot: CD53 Antibody (004) [NBP2-90697] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate2 uL anti-Mouse CD53 Antibody (004) and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-Mouse Mouse CD53 Antibody (004),at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 24 kDa Observed band size: 24 kDa](http://images.novusbio.com/fullsize/CD53-Antibody-004-Western-Blot-NBP2-90697-img0003.jpg "Western Blot: CD53 Antibody (004) [NBP2-90697] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate2 uL anti-Mouse CD53 Antibody (004) and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-Mouse Mouse CD53 Antibody (004),at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 24 kDa Observed band size: 24 kDa")
![Western Blot: CD53 Antibody (004) [NBP2-90697] - Anti-CD53 rabbit monoclonal antibody at 1:500 dilutionLane A: Raji Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/CD53-Antibody-004-Western-Blot-NBP2-90697-img0002.jpg)
![Western Blot: CD53 Antibody (104) [NBP2-90952] - Anti-CD53 rabbit monoclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted and observed band size: 34 kDa.](http://images.novusbio.com/fullsize/CD53-Antibody-104-Western-Blot-NBP2-90952-img0004.jpg "Western Blot: CD53 Antibody (104) [NBP2-90952] - Anti-CD53 rabbit monoclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted and observed band size: 34 kDa.")
![Immunoprecipitation: CD53 Antibody (104) [NBP2-90952] - CD53 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate. Lane B:0.5 mg Raji Whole Cell Lysate. 2 uL anti-CD53 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-cyno CD53 rabbit monoclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted and observed band size: 34 kDa.](http://images.novusbio.com/fullsize/CD53-Antibody-104-Immunoprecipitation-NBP2-90952-img0003.jpg)
