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CD5 Antibody (205919) [Unconjugated]

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Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD5 Monoclonal Antibody (Catalog # MAB1636, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
205919
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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CD5 Antibody (205919) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD5
Arg25-Asn371
Accession # P06127
Specificity
Detects human CD5 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CD5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB1636 in the following applications:

Publications
Read Publications using
MAB1636 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD5 Antibody (205919) [Unconjugated]

  • CD5 antigen (p56-62)
  • CD5 antigen
  • CD5 molecule
  • CD5
  • LEU1T-cell surface glycoprotein CD5
  • Lymphocyte antigen T1/Leu-1
  • T1

Background

CD5, also known as Leu-1, is a type I transmembrane protein belonging to the scavenger receptor cysteine-rich (SRCR) superfamily. It is expressed on thymocytes, T cells and the B1a subpopulation of B cells. CD5 binds the C-type lectin, CD72, and modulates signaling through the antigen receptors on T and B cells. CD5 has been shown to provide either positive or negative co-stimulatory signals depending on cell type to regulate immune responses.

  1. Ledbetter, J.A. et al. (1985) J. Immunol. 135:2331.
  2. Damle, N.K. et al. (1988) J. Immunol. 140:1753.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD5 Antibody (MAB1636)(3)

Review for CD5 Antibody (MAB1636) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Canine.

Reviews using MAB1636:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Canine
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry CD5 MAB1636
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4
reviewed by:
A G
Flow Canine 03/13/2017
View

Summary

ApplicationFlow Cytometry
Sample TestedWhole blood cells
SpeciesCanine
LotMAB1636

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD5 Antibody (MAB1636) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CD5 Products

Blogs on CD5.

How To Identify B Cell Subsets Using Flow Cytometry
By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified...  Read full blog post.

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Recent Reviews

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A G
03/13/2017
Application: Flow
Species: Canine

Bioinformatics

Gene Symbol CD5
Uniprot