CD300f/LMIR3/CD300LF Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

CD300f, also known as CD300LF, LMIR3, IREM-1, CLM-1, IgSF13, DIgR1, and MAIR-V, is a 50‑60 kDa glycoprotein member of the immunoglobulin superfamily (1). Human CD300f consists of a 137 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 113 aa cytoplasmic domain that contains multiple immunoreceptor tyrosine-based inhibitory motifs (ITIMs) or ITIM-like sequences (2, 3). Alternate splicing generates additional isoforms that carry substituted C-terminal tails of varying lengths and sequences following the ECD or transmembrane segment (3). Within the ECD, human CD300f shares 43% aa sequence identity with mouse and rat CD300f. CD300f is expressed on the surface of dendritic cells, monocytes, granulocytes, and mast cells as well as on acute myeloid leukemia (AML) blasts (2‑4). Pervanadate treatment or antibody crosslinking of CD300f induces phosphorylation of tyrosine residues in the cytoplasmic domain and the subsequent recruitment of phosphatases SHIP, SHP-1, SHP-2, and the p85 alpha subunit of PI3K (2, 3, 5, 6). CD300f ligation can induce cell death and inhibit signaling through multiple receptors including Fc epsilon RI, LMIR4, SCF R, TLR2, TLR3, and TLR9 (3‑8). In contrast, it enhances TLR4-mediated signaling/cytokine production in mast cells through association with the activating signaling protein FcR gamma (5). In mouse, a splice variant of CD300f (known as DIgR2, with a 7 aa insertion in the ECD) inhibits CD4+ T cell activation and in vivo Th1 and CTL responses (9). CD300f is up‑regulated on monocytes surrounding experimentally-induced spinal cord demyelination and functions as a negative regulator of inflammation in the CNS (10).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Cell Culture, Flow Cytometry, Immunoprecipitation, Neutralization.

Showing Publications 1 - 4 of 4.

Publications using AF2774	Applications	Species
VR Graziano, FC Walker, EA Kennedy, J Wei, K Ettayebi, MS Strine, RB Filler, E Hassan, LL Hsieh, AS Kim, AO Kolawole, CE Wobus, LC Lindesmith, RS Baric, MK Estes, RC Orchard, MT Baldridge, CB Wilen CD300lf is the primary physiologic receptor of murine norovirus but not human norovirus PLoS Pathog., 2020-04-06;16(4):e1008242. 2020-04-06 [PMID: 32251490] (Cell Culture, Human)	Cell Culture	Human
Kei Haga, Akira Fujimoto, Reiko Takai-Todaka, Motohiro Miki, Yen Hai Doan, Kosuke Murakami et al. Functional receptor molecules CD300lf and CD300ld within the CD300 family enable murine noroviruses to infect cells Proceedings of the National Academy of Sciences 2016-10-11 [PMID: 27681626]
Izawa K, Isobe M, Matsukawa T, Ito S, Maehara A, Takahashi M, Yamanishi Y, Kaitani A, Oki T, Okumura K, Kitamura T, Kitaura J Sphingomyelin and ceramide are physiological ligands for human LMIR3/CD300f, inhibiting FcepsilonRI-mediated mast cell activation. J Allergy Clin Immunol, 2013-09-12;133(1):270-3.e1-7. 2013-09-12 [PMID: 24035150] (Flow Cytometry, Neutralization, Human)	Flow Cytometry, Neutralization	Human
Asai , Tsuneaki, Morrison , Sherie L The SRC family tyrosine kinase HCK and the ETS family transcription factors SPIB and EHF regulate transcytosis across a human follicle-associated epithelium model. J Biol Chem, 2013-02-25;288(15):10395-405. 2013-02-25 [PMID: 23439650] (Immunoprecipitation, Human)	Immunoprecipitation	Human

Array AF2774

• CD300f/LMIR3/CD300LF Antibodies
• CD300f/LMIR3/CD300LF ELISA Kits
• CD300f/LMIR3/CD300LF Lysate
• CD300f/LMIR3/CD300LF Protein
• CD300f/LMIR3/CD300LF Proteins and Enzymes