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Recombinant Human CD3 epsilon His Protein

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SDS-Page: Recombinant Human CD3 epsilon His Protein [NBP2-22752] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
SDS-Page: Recombinant Human CD3 epsilon His Protein [NBP2-22752] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

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Recombinant Human CD3 epsilon His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 23-126 of Human CD3 epsilon

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSDGNEEMG GITQTPYKVS ISGTTVILTC PQYPGSEILW QHNDKNIGGD EDDKNIGSDE DHLSLKEFSE LEQSGYYVCY PRGSKPEDAN FYLYLRARVC ENCMEMD
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CD3E
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
14.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-22752 in the following application:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human CD3 epsilon His Protein

  • CD3 epsilon
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3e
  • CD3-epsilon
  • FLJ18683
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Background

CD3E is the CD3-epsilonpolypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. Recombinant human CD3E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for CD3 epsilon Recombinant Protein (NBP2-22752) (0)

There are no publications for CD3 epsilon Recombinant Protein (NBP2-22752).
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Review for CD3 epsilon Recombinant Protein (NBP2-22752) (1) 41

Average Rating: 4
(Based on 1 review)

Reviews using NBP2-22752:
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Binding assay/Protein-protein interaction
(1)
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  4
reviewed by:
Bum-Yeol Hwang
 Binding assay/Protein-protein interaction 06/03/2020
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Summary

ApplicationBinding assay/Protein-protein interaction
Lot19186501

FAQs for CD3 epsilon Recombinant Protein (NBP2-22752). (Showing 1 - 1 of 1 FAQ).

  1. I have a question about recombinant Human CD3 epsilon Protein (Cat # NBP2-22752: do you have gel images from non-reducing gel? Does the protein solution contain aggregates?
    • We do not have an image in non-reducing conditions. As for aggregation, it has a hydrophobic region, so it may have some aggregation when boiled. Therefore, we recommend heating to 37C rather than boiling when loading.

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Recent Reviews

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 Bum-Yeol Hwang
06/03/2020
Application: Binding assay/Protein-protein interaction
Species:
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Bioinformatics

Gene Symbol CD3E