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CD2 Antibody (299812)

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CD2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human CD2 Monoclonal Antibody (Catalog # MAB18561) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human CD2 Monoclonal Antibody (Catalog # MAB18561) for 3 hours at room temperature. Cells were stained with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
299812
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB18561U-100UG
Alexa Fluor 405
FAB18561V-100UG
Alexa Fluor 488
FAB18561G-100UG
Alexa Fluor 532
FAB18561X
Alexa Fluor 594
FAB18561T-100UG
Alexa Fluor 647
FAB18561R-100UG
Alexa Fluor 700
FAB18561N-100UG
Alexa Fluor 750
FAB18561S-100UG
Allophycocyanin
FAB18561A
Allophycocyanin/Cy7
FAB18561APCCY7
Biotin
FAB18561B
CoraFluor 1
FAB18561CL1
DyLight 350
FAB18561D
DyLight 405
FAB18561E
DyLight 488
FAB18561K
DyLight 550
FAB18561L
DyLight 594
FAB18561M
DyLight 650
FAB18561W
DyLight 680
FAB18561Y
DyLight 755
FAB18561Z
HRP
FAB18561H
Janelia Fluor 525
FAB18561JF525
Janelia Fluor 549
FAB18561I
Janelia Fluor 585
FAB18561JF585
Janelia Fluor 635
FAB18561JF635
Janelia Fluor 646
FAB18561J
Janelia Fluor 669
FAB18561JF669
PE/Atto594
FAB18561PEATT594
PE/Cy5.5
FAB18561PECY55
PE/Cy7
FAB18561PECY7
PerCP
FAB18561C
mFluor Violet 450 SE
FAB18561MFV450
mFluor Violet 500 SE
FAB18561MFV500
mFluor Violet 610 SE
FAB18561MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB18561A
Carrier Free
MAB18561-SP
Carrier Free
FAB18561V-100UG
Carrier Free
FAB18561U-100UG
Carrier Free
FAB18561T-100UG
Carrier Free
FAB18561S-100UG
Carrier Free
FAB18561R-100UG
Carrier Free
FAB18561N-100UG
Carrier Free
FAB18561G-100UG
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CD2 Antibody (299812) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD2
Lys25-Asp209
Accession # P06729
Specificity
Detects recombinant human CD2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) CD2 or rmCD2F-10 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD2 Antibody (299812)

  • CD2 antigen (p50), sheep red blood cell receptor
  • CD2 antigen
  • CD2 molecule
  • CD2
  • Erythrocyte receptor
  • FLJ46032
  • LFA-2
  • LFA-3 receptor
  • lymphocyte-function antigen-2
  • Rosette receptor
  • SRBC
  • T11
  • T-cell surface antigen CD2
  • T-cell surface antigen T11/Leu-5

Background

CD2 is a type I glycoprotein belonging to the Ig superfamily and is expressed on T, NK, B, and some antigen presenting cells. Human CD2 binds human CD58 on antigen-presenting cells to induce co-stimulating signals in T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD2
Uniprot