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CD151 Antibody (11G5a) - Azide and BSA Free

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Immunohistochemistry-Paraffin: CD151 Antibody (11G5a) - Azide and BSA Free [NBP3-08797] - Formalin-fixed, paraffin-embedded human colon stained with CD151 Mouse Monoclonal Antibody (11G5a).
Immunohistochemistry-Paraffin: CD151 Antibody (11G5a) - Azide and BSA Free [NBP3-08797] - Formalin-fixed, paraffin-embedded human colon stained with CD151 Mouse Monoclonal Antibody (11G5a).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
11G5a
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08797AF350
Alexa Fluor 405
NBP3-08797AF405
Alexa Fluor 488
NBP3-08797AF488
Alexa Fluor 532
NBP3-08797AF532
Alexa Fluor 594
NBP3-08797AF594
Alexa Fluor 647
NBP3-08797AF647
Alexa Fluor 700
NBP3-08797AF700
Alexa Fluor 750
NBP3-08797AF750
Allophycocyanin
NBP3-08797APC
Biotin
NBP3-08797B
CoraFluor 1
NBP3-08797CL1
DyLight 350
NBP3-08797UV
DyLight 405
NBP3-08797V
DyLight 488
NBP3-08797G
DyLight 550
NBP3-08797R
DyLight 594
NBP3-08797DL594
DyLight 650
NBP3-08797C
DyLight 680
NBP3-08797FR
DyLight 755
NBP3-08797IR
FITC
NBP3-08797F
HRP
NBP3-08797H
Janelia Fluor 525
NBP3-08797JF525
Janelia Fluor 549
NBP3-08797JF549
Janelia Fluor 646
NBP3-08797JF646
Janelia Fluor 669
NBP3-08797JF669
PE
NBP3-08797PE
PerCP
NBP3-08797PCP
Unconjugated
NBP3-07476-100ug
mFluor Violet 450 SE
NBP3-08797MFV450
mFluor Violet 500 SE
NBP3-08797MFV500
mFluor Violet 610 SE
NBP3-08797MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-07476-100ug
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CD151 Antibody (11G5a) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07476).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
The details of the immunogen for this antibody are not available.
Localization
Cell Surface
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD151
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for CD151 Antibody (11G5a) - Azide and BSA Free

  • CD151 antigen (Raph blood group)
  • CD151 antigenplatelet surface glycoprotein gp27
  • CD151 molecule (Raph blood group)
  • CD151
  • GP27
  • Membrane glycoprotein SFA-1
  • MER2
  • PETA3
  • PETA-3
  • PETA-3SFA-1
  • platelet-endothelial cell tetraspan antigen 3
  • Platelet-endothelial tetraspan antigen 3
  • RAPH
  • SFA1
  • SFA-1
  • tetraspanin-24
  • TSPAN24
  • tspan-24
  • TSPAN24hemidesmosomal tetraspanin CD151

Background

CD151 is essential for the proper assembly of the glomerular and tubular basement membranes in kidney. It interacts with integrins alpha3beta1, alpha5beta1, alpha3beta1 and alpha6beta4, and with CD9 and CD181. It is expressed in a variety of tissues including vascular endothelium and epidermis, platelets, monocytes and also erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes. CD151 is however not expressed by lymphocytes or granulocytes. Defects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD151