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Mouse CD117/c-kit ELISA Kit (Colorimetric)

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ELISA: Mouse CD117/c-kit ELISA Kit (Colorimetric) [NBP3-06959] - Samples were spiked with high concentrations of Mouse CD117/c-kit and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Mouse CD117/c-kit ELISA Kit (Colorimetric) [NBP3-06959] - Samples were spiked with high concentrations of Mouse CD117/c-kit and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Mouse CD117/c-kit ELISA Kit (Colorimetric) [NBP3-06959] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL

Order Details

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Mouse CD117/c-kit ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse CD117/c-kit in samples. No significant cross-reactivity or interference between Mouse CD117/c-kit and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.33
Intra-Assay
4.58
Spike Recovery
87-109 %
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
KIT

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse CD117/c-kit ELISA Kit (Colorimetric)

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Blogs on CD117/c-kit.

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Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene ...  Read full blog post.
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Product PDFs

  SDS
  Mouse CD117/c-kit ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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