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CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free

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Western Blot: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIT ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIT (1:2000). Positive lysates.
Western Blot: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIT ( Right lane) cDNA for 48 hrs and lysed. ...read more
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of Human colon tissue within the normal limits using anti-KIT mouse monoclonal antibody. (Heat-induced epitope ...read more
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of Adenocarcinoma of Human colon tissue using anti-KIT mouse monoclonal antibody. (Heat-induced epitope ...read more
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KIT mouse monoclonal antibody. ...read more
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of Carcinoma of Human thyroid tissue using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval ...read more
Immunohistochemistry-Paraffin: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Staining of Human lymphoma tissue using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA ...read more
Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Carcinoma of Human bladder tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for ...read more
Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 ...read more
Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C ...read more
Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free [NBP2-71076] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
OTI2B12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71076AF350
Alexa Fluor 405
NBP2-71076AF405
Alexa Fluor 488
NBP2-71076AF488
Alexa Fluor 532
NBP2-71076AF532
Alexa Fluor 594
NBP2-71076AF594
Alexa Fluor 647
NBP2-71076AF647
Alexa Fluor 700
NBP2-71076AF700
Alexa Fluor 750
NBP2-71076AF750
Allophycocyanin
NBP2-71076APC
Biotin
NBP2-71076B
CoraFluor 1
NBP2-71076CL1
DyLight 350
NBP2-71076UV
DyLight 405
NBP2-71076V
DyLight 488
NBP2-71076G
DyLight 550
NBP2-71076R
DyLight 594
NBP2-71076DL594
DyLight 650
NBP2-71076C
DyLight 680
NBP2-71076FR
DyLight 755
NBP2-71076IR
FITC
NBP2-71076F
HRP
NBP2-71076H
Janelia Fluor 525
NBP2-71076JF525
Janelia Fluor 549
NBP2-71076JF549
Janelia Fluor 646
NBP2-71076JF646
Janelia Fluor 669
NBP2-71076JF669
PE
NBP2-71076PE
PerCP
NBP2-71076PCP
Unconjugated
NBP2-45508
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45508
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CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 27-308 of human KIT(NP_000213) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
KIT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
109.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD117/c-kit
Antibody (OTI2B12) - ...
NBP2-71076
CD117/c-kit Antibody (OTI2B12) - Azide and BSA Free
Species: Hu
Applications: WB, IHC

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Secondary Antibodies

 

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Blogs on CD117/c-kit.

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Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene ...  Read full blog post.
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Bioinformatics

Gene Symbol KIT