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CD117/c-kit Antibody (ACK2) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, GpSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, IHC
Clone
ACK2
Clonality
Monoclonal
Host
Rat
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43359AF350
Alexa Fluor 405
NBP1-43359AF405
Alexa Fluor 488
NBP1-43359AF488
Alexa Fluor 532
NBP1-43359AF532
Alexa Fluor 594
NBP1-43359AF594
Alexa Fluor 647
NBP1-43359AF647
Alexa Fluor 700
NBP1-43359AF700
Alexa Fluor 750
NBP1-43359AF750
Allophycocyanin
NBP1-43359APC
Allophycocyanin/Cy7
NBP1-43359APCCY7
Biotin
NBP1-43359B
DyLight 350
NBP1-43359UV
DyLight 405
NBP1-43359V
DyLight 488
NBP1-43359G
DyLight 550
NBP1-43359R
DyLight 594
NBP1-43359DL594
DyLight 650
NBP1-43359C
DyLight 680
NBP1-43359FR
DyLight 755
NBP1-43359IR
FITC
NBP1-43359F
HRP
NBP1-43359H
Janelia Fluor 525
NBP1-43359JF525
Janelia Fluor 549
NBP1-43359JF549
Janelia Fluor 646
NBP1-43359JF646
Janelia Fluor 669
NBP1-43359JF669
PE
NBP1-43359PE
PE/Atto594
NBP1-43359PEATT594
PE/Cy5.5
NBP1-43359PECY55
PE/Cy7
NBP1-43359PECY7
PerCP
NBP1-43359PCP
Unconjugated
NBP1-43359-0.1mg
mFluor Violet 450 SE
NBP1-43359MFV450
mFluor Violet 500 SE
NBP1-43359MFV500
mFluor Violet 610 SE
NBP1-43359MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-43359-0.1mg
BSA Free
NBP1-43359-0.1mg
BSA Free
NBP1-43359-0.05mg
BSA Free
NBP1-43359-0.025mg
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CD117/c-kit Antibody (ACK2) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Mouse CD117
Marker
Hematopoietic Stem Cell Marker
Specificity
The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
KIT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23967247). Guinea Pig reactivity reported in scientific literature (PMID: 21654812)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for CD117/c-kit Antibody (ACK2) [CoraFluor™ 1]

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-43359CL1
CD117/c-kit Antibody (ACK2) [CoraFluor™ 1]
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Applications: WB, Flow, ICC/IF, IHC, IP, IHC

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Secondary Antibodies

 

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Blogs on CD117/c-kit.

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Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene ...  Read full blog post.
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Bioinformatics

Gene Symbol KIT