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CCR6 Antibody (876515R) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with either (A) rat CCR6 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Rat CCR6 Monoclonal Antibody (Catalog # MAB8320R) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2b isotype control antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with either (A) rat CCR6 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Rat CCR6 Monoclonal Antibody (Catalog # MAB8320R) followed by APC-conjugated ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, CyTOF-ready
Clone
876515R
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB8320RU-100UG
Alexa Fluor 405
FAB8320RV-100UG
Alexa Fluor 488
FAB8320RG-100UG
Alexa Fluor 532
FAB8320RX
Alexa Fluor 594
FAB8320RT-100UG
Alexa Fluor 647
FAB8320RR-100UG
Alexa Fluor 700
FAB8320RN-100UG
Alexa Fluor 750
FAB8320RS-100UG
Allophycocyanin
FAB8320RA
Allophycocyanin/Cy7
FAB8320RAPCCY7
Biotin
FAB8320RB
CoraFluor 1
FAB8320RCL1
DyLight 350
FAB8320RD
DyLight 405
FAB8320RE
DyLight 488
FAB8320RK
DyLight 550
FAB8320RL
DyLight 594
FAB8320RM
DyLight 650
FAB8320RW
DyLight 680
FAB8320RY
DyLight 755
FAB8320RZ
FITC
FAB8320RF
HRP
FAB8320RH
Janelia Fluor 525
FAB8320RJF525
Janelia Fluor 549
FAB8320RI
Janelia Fluor 646
FAB8320RJ
Janelia Fluor 669
FAB8320RJF669
PE
FAB8320RP
PE/Atto594
FAB8320RPEATT594
PE/Cy5.5
FAB8320RPECY55
PE/Cy7
FAB8320RPECY7
PerCP
FAB8320RC
mFluor Violet 450 SE
FAB8320RMFV450
mFluor Violet 500 SE
FAB8320RMFV500
mFluor Violet 610 SE
FAB8320RMFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8320R-SP
Carrier Free
FAB8320RU-100UG
Carrier Free
FAB8320RR-100UG
Carrier Free
FAB8320RN-100UG
Carrier Free
FAB8320RV-100UG
Carrier Free
FAB8320RT-100UG
Carrier Free
FAB8320RS-100UG
Carrier Free
FAB8320RG-100UG
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CCR6 Antibody (876515R) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
NS0 mouse myeloma cell line transfected with rat CCR6
Met1-Met366
Accession # Q5BK58
Specificity
Detects rat CCR6 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR6 Antibody (876515R) [Unconjugated]

  • BN-1
  • CC-CKR-6
  • CCR6
  • CCR-6
  • CD196 antigen
  • CD196
  • chemokine (C-C motif) receptor 6
  • CKRL3
  • CKR-L3
  • CKR-L3G-protein coupled receptor 29
  • CKRL3LARC receptor
  • CMKBR6
  • CMKBR6STRL22
  • DRY6
  • GPR29
  • GPR29C-C CKR-6
  • GPRCY4
  • GPR-CY4
  • GPRCY4DRY-6
  • lymphocyte, 22
  • STRL22

Background

CCR6 is an approximately 45 kDa G-protein linked seven transmembrane domain receptor that binds the chemokine CCL20/MIP-3 alpha . Among hematopoietic cells, CCR6 is expressed on B cells, immature dendritic cells, neutrophils, and various T cell subsets (e.g. CD4+ T cells, regulatory T cells, effector memory Th17 cells, and g/d T cells). It is upregulated in several cancers, Alzheimer’s brain, and the airways in obstructive pulmonary disease. CCR6 plays an important role in mucosal immunity, IgE mediated allergic reactions, immune cell infiltration into sites of inflammation, and tumor cell invasiveness. It also promotes chemotaxis and tumor angiogenesis in response to beta defensins. Rat CCR6 shares 74% and 87% amino acid sequence identity with human and mouse CCR6, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCR6 Antibody
(876515R) [Unconjugat
MAB8320R
CCR6 Antibody (876515R) [Unconjugated]
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