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CCR5 Antibody (45549) [Unconjugated]

 (7 Publications)
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CCR5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CCR5 Monoclonal Antibody (Catalog # MAB183) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CCR5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CCR5 Monoclonal Antibody (Catalog # MAB183) at 8 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
45549
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB183-SP
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CCR5 Antibody (45549) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human CCR5
Met1-Leu352
Accession # P51681
Specificity
Detects human CCR5.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CCR5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB183 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR5 Antibody (45549) [Unconjugated]

  • C-C CKR-5
  • C-C motif chemokine receptor 5 A159A
  • CCCKR5
  • CC-CKR-5FLJ78003
  • CCR5
  • CCR-5
  • CD195 antigen
  • CD195
  • chemokine (C-C motif) receptor 5
  • chemokine receptor CCR5
  • chemr13
  • CKR5
  • CKR-5
  • HIV-1 fusion coreceptor
  • IDDM22CMKBR5C-C chemokine receptor type 5

Background

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha , MIP-1 beta , MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCR5 Antibody
(45549) [Unconjugated
MAB183
CCR5 Antibody (45549) [Unconjugated]
Species: Hu
Applications: ICC/IF

Publications for CCR5 Antibody (MAB183)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Bioassay, Flow Cytometry, ICC.


Filter By Application
Bioassay
(1)
Flow Cytometry
(4)
ICC
(1)
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Human
(6)
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Showing Publications 1 - 7 of 7.
Publications using MAB183 Applications Species
Shengli Song, Miriam Manook, Jean Kwun, Annette M. Jackson, Stuart J. Knechtle, Garnett Kelsoe A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines Communications Biology 2021-11-25 [PMID: 34824350]
Nakano Y, Monde K, Terasawa H, Yuan Y, Yusa K, Harada S, Maeda Y Preferential recognition of monomeric CCR5 expressed in cultured cells by the HIV-1 envelope glycoprotein gp120 for the entry of R5 HIV-1. Virology, 2014-01-31;452(0):117-24. 2014-01-31 [PMID: 24606688] (Flow Cytometry, Human) Flow Cytometry Human
Alonzo , Francis, Kozhaya , Lina, Rawlings , Stephen, Reyes-Robles , Tamara, DuMont , Ashley L, Myszka , David G, Landau , Nathanie, Unutmaz , Derya, Torres , Victor J CCR5 is a receptor for Staphylococcus aureus leukotoxin ED. Nature, 2012-12-12;493(7430):51-5. 2012-12-12 [PMID: 23235831] (Bioassay, Human) Bioassay Human
Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions. Int. J. Cancer, 2008-02-15;122(4):769-76. 2008-02-15 [PMID: 17935139] (Flow Cytometry, Human) Flow Cytometry Human
Pulliam L, Sun B, Rempel H Invasive chronic inflammatory monocyte phenotype in subjects with high HIV-1 viral load. J. Neuroimmunol., 2004-12-01;157(1):93-8. 2004-12-01 [PMID: 15579285] (ICC, Human) ICC Human
Ottonello L, Montecucco F, Bertolotto M, Arduino N, Mancini M, Corcione A, Pistoia V, Dallegri F CCL3 (MIP-1alpha) induces in vitro migration of GM-CSF-primed human neutrophils via CCR5-dependent activation of ERK 1/2. Cell. Signal., 2005-03-01;17(3):355-63. 2005-03-01 [PMID: 15567066] (Flow Cytometry, Human) Flow Cytometry Human
Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function. J. Biol. Chem., 1999-04-02;274(14):9617-26. 1999-04-02 [PMID: 10092648] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol CCR5
Uniprot