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Recombinant Rat CCL7/MCP-3/MARC Protein

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SDS-Page: Rat CCL7/MCP-3/MARC Protein [NBP2-35267]
SDS-Page: Rat CCL7/MCP-3/MARC Protein [NBP2-35267]

Product Details

Summary
Reactivity RtSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-35267-10ug
Carrier-Free
NBP2-35267-1mg
Carrier-Free
NBP2-35267-500ug
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Recombinant Rat CCL7/MCP-3/MARC Protein Summary

Description
A single non-glycosylated polypeptide chain containing 74 amino acids corresponding to CCL7/MCP-3/MARC Source: E. coli

Uniprot ID: Q9QXY8

Amino Acid Sequence: QPDGTNSSTC CYVKKQKIPK RNLKSYRKIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTS TPKP
Details of Functionality
MCP3 Protein is fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CCL7
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of CCL7/MCP-3/MARC as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
8.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Rat CCL7/MCP-3/MARC Protein

  • C-C motif chemokine 7
  • CCL7
  • chemokine (C-C motif) ligand 7
  • FIC
  • MARC
  • MCP-3
  • MCP-3/CCL7
  • MCP3Monocyte chemotactic protein 3
  • MCP-3Small-inducible cytokine A7
  • MGC138465
  • Monocyte chemoattractant protein 3
  • NC28SCYA6
  • SCYA7MGC138463
  • small inducible cytokine A7 (monocyte chemotactic protein 3)

Background

Rat CCL7, belonging to the CC chemokine family, is also named as MCP-3, because the sequence comparison suggested that the rat CCL7 is a homologue of the human MCP-3 and is postulated the rat MCP-3. The MCP-3 protein family signals through CCR2 expect MCP-1 and possess cross-reacts across species. CCL7/MCP3 has chemotactic functions for monocytes and eosinophils, but not for neutrophils. Additionally, it can activated NK cells as well as CD4+ and CD8+ T lymphocytes. CCL7 was found to interact with MMP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CCL7