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CCL7/MCP-3/MARC Antibody [Unconjugated]

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Recombinant Human CCL7/MCP‑3 (Catalog # 282‑P3) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human CCL7/MCP‑3 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL7/MCP‑3 Polyclonal Antibody (Catalog # AB-282-NA). The ND50 is typically 30-60 µg/mL.
Recombinant Human CCL7/MCP‑3 (Catalog # 282‑P3) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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CCL7/MCP-3/MARC Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CCL7/MCP‑3/MARC
Gln24-109
Accession # P80098
Specificity
Detects human CCL7/MCP‑3/MARC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 6% cross-reactivity with recombinant human (rh) MCP-2 and rhMCP-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL7
Purity Statement
Protein A or G purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 30-60 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
AB-282-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL7/MCP-3/MARC Antibody [Unconjugated]

  • C-C motif chemokine 7
  • CCL7
  • chemokine (C-C motif) ligand 7
  • FIC
  • MARC
  • MCP-3
  • MCP3Monocyte chemotactic protein 3
  • MCP-3Small-inducible cytokine A7
  • MGC138465
  • Monocyte chemoattractant protein 3
  • NC28SCYA6
  • SCYA7MGC138463
  • small inducible cytokine A7 (monocyte chemotactic protein 3)

Background

MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2.

CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites.

Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL7/MCP-3/MARC Antibody (AB-282-NA)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Neutralization.


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Bioinformatics

Gene Symbol CCL7
Uniprot