Reactivity | MuSpecies Glossary |
Applications | WB, B/N, ELISA(Cap) |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Unconjugated |
Concentration | LYOPH |
Immunogen | E. coli-derived recombinant mouse CCL2/JE/MCP-1 Gln24-Arg96 Accession # Q5SVU3 |
Specificity | Detects mouse CCL2/JE/MCP-1 in ELISAs and Western blots. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | Ccl2 |
Purity Statement | Antigen Affinity-purified |
Endotoxin Note | <0.10 EU per 1 μg of the antibody by the LAL method. |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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Application Notes | ELISA Capture: Mouse CCL2/JE/MCP-1 Antibody (Catalog # AF-479-NA) ELISA Detection:Mouse CCL2/JE/MCP-1 Biotinylated Antibody (Catalog # BAF479) Standard: Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE) |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.
Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
IHC-P | Mouse | 01/08/2015 |
Summary
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reviewed by:
Verified Customer |
IHC-Fr | Mouse | 01/08/2015 |
Summary
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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Verified Customer 01/08/2015 |
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Application: | IHC-P | |
Species: | Mouse |
Verified Customer 01/08/2015 |
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Application: | IHC-Fr | |
Species: | Mouse |