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CCL19/MIP-3 beta Antibody [Unconjugated]

 (15 Publications)
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Recombinant Mouse CCL19/MIP‑3 beta (Catalog #440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line).The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP‑3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880). The ND50 is typically 0.8-4 μg/mL.
Recombinant Mouse CCL19/MIP‑3 beta (Catalog #440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line).The amount of cells that migrated through ...read more
CCL19/MIP-3 beta was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. ...read more
CCL19/MIP-3 beta was detected in immersion fixed mouse dendritic cells using Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF880
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF880-SP
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CCL19/MIP-3 beta Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL19/MIP-3 beta
Gly26-Val107 (Ser108LeuGlu)
Accession # Q548P0
Specificity
Detects mouse CCL19/MIP-3 beta in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Ccl19
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.8-4 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF880 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL19/MIP-3 beta Antibody [Unconjugated]

  • beta chemokine exodus-3
  • Beta-chemokine exodus-3
  • CC chemokine ligand 19
  • C-C motif chemokine 19
  • CCL19
  • chemokine (C-C motif) ligand 19
  • CKb11
  • EBI1-ligand chemokine
  • ELC
  • ELCMIP-3-beta
  • Epstein-Barr virus-induced molecule 1 ligand chemokine
  • exodus-3
  • Macrophage inflammatory protein 3 beta
  • macrophage inflammatory protein 3-beta
  • MGC34433
  • MIP3 beta
  • MIP-3 beta
  • MIP-3b
  • MIP3BCK beta-11
  • SCYA19EBI1 ligand chemokine
  • small inducible cytokine subfamily A (Cys-Cys), member 19
  • Small-inducible cytokine A19

Background

CCL19/MIP-3 beta , also known as ELC (EBI1-Ligand Chemokine), is a reported beta  chemokine that binds specifically to the chemokine receptor CCR-7/EBI-1/BLR-2. Mouse (human) MIP-3 beta cDNA encodes a 108 (98) amino acid residue precursor protein with a predicted 25 (21) aa residue signal peptide that is cleaved to form the 83 (77) aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta . MIP-3 beta  has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T cell zone. The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta has been shown to be chemotactic for T cells and B cells. The MIP-3 beta receptor (CCR7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpes virus 6 or 7.

  1. Kim, C.H. et al. (1998) J. Immunol. 160:2418.
  2. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
  3. Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
  4. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL19/MIP-3 beta Antibody (AF880)(15)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Flow Cytometry, IHC, IHC-Fr, IHC-P, Neutralization.


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Flow Cytometry
(2)
IHC
(2)
IHC-Fr
(4)
IHC-P
(1)
Neutralization
(3)
All Applications
Filter By Species
Mouse
(11)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF880 Applications Species
J Liu, Y Cheng, X Zhang, Y Chen, H Zhu, K Chen, S Liu, Z Li, X Cao Glycosyltransferase Extl1 promotes CCR7-mediated dendritic cell migration to restrain infection and autoimmunity Cell Reports, 2023-01-18;42(1):111991. 2023-01-18 [PMID: 36656709] (Flow Cytometry, Mouse) Flow Cytometry Mouse
V Saxena, W Piao, L Li, C Paluskievi, Y Xiong, T Simon, R Lakhan, CC Brinkman, S Walden, KL Hippen, M WillsonShi, YS Lee, C Wagner, BR Blazar, JS Bromberg Treg tissue stability depends on lymphotoxin beta-receptor- and adenosine-receptor-driven lymphatic endothelial cell responses Cell Reports, 2022-04-19;39(3):110727. 2022-04-19 [PMID: 35443187] (IHC, Mouse) IHC Mouse
Juana Serrano-Lopez, Shailaja Hegde, Sachin Kumar, Josefina Serrano, Jing Fang, Ashley M Wellendorf et al. Inflammation rapidly recruits mammalian GMP and MDP from bone marrow into regional lymphatics eLife 2021-04-08 [PMID: 33830019]
M Chen, RR Reed, AP Lane Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense Cell Stem Cell, 2019-09-12;25(4):501-513.e5. 2019-09-12 [PMID: 31523027] (IHC-Fr, Neutralization, Mouse) IHC-Fr, Neutralization Mouse
Alice E. Denton, Edward J. Carr, Lukasz P. Magiera, Andrew J.B. Watts, Douglas T. Fearon Embryonic FAP+ lymphoid tissue organizer cells generate the reticular network of adult lymph nodes Journal of Experimental Medicine 2019-10-07 [PMID: 31324739]
AY Nossent, AJ Bastiaanse, EA Peters, MR de Vries, Z Aref, SM Welten, SC de Jager, TC van der Po, PH Quax CCR7-CCL19/CCL21 Axis is Essential for Effective Arteriogenesis in a Murine Model of Hindlimb Ischemia J Am Heart Assoc, 2017-03-08;6(3):. 2017-03-08 [PMID: 28275068] (IHC-Fr, Mouse) IHC-Fr Mouse
Yuki Sato, Akiko Mii, Yoko Hamazaki, Harumi Fujita, Hirosuke Nakata, Kyoko Masuda et al. Heterogeneous fibroblasts underlie age-dependent tertiary lymphoid tissues in the kidney JCI Insight 2016-10-05 [PMID: 27699223]
Jing Hua, William Stevenson, Thomas H. Dohlman, Takenori Inomata, Maryam Tahvildari, Narghes Calcagno et al. Graft Site Microenvironment Determines Dendritic Cell Trafficking Through the CCR7-CCL19/21 Axis Investigative Opthalmology & Visual Science 2016-04-01 [PMID: 27031839]
Ibrahim M, Barnes J, Osorio E, Anstead G, Jimenez F, Osterholzer J, Travi B, Ahuja S, White A, Melby P Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection. Infect Immun, 2014-05-12;82(8):3098-112. 2014-05-12 [PMID: 24818662] (IHC, Mouse) IHC Mouse
Jimenez F, Quinones MP, Martinez HG, Estrada CA, Clark K, Garavito E, Ibarra J, Melby PC, Ahuja SS CCR2 plays a critical role in dendritic cell maturation: possible role of CCL2 and NF-kappa B. J. Immunol., 2010-04-19;184(10):5571-81. 2010-04-19 [PMID: 20404272] (Neutralization, Mouse) Neutralization Mouse
Show All 15 Publications.

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Bioinformatics

Gene Symbol Ccl19
Uniprot