Mouse Cathepsin L ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Mouse CTSL in samples. No Significant cross-reactivity or interference between Mouse CTSL and analogues was observed. |
Standard Curve Range |
31.25-2000 pg/mL |
Sensitivity |
18.75 pg/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
CV% < 43.4 |
Intra-Assay |
CV% < 410 |
Spike Recovery |
89-105% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
CTSL |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Mouse Cathepsin L ELISA Kit (Colorimetric)
Background
Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumour invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intralysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive proform, and mRNA expression level seems to correlate with their metastatic potential.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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