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Cathepsin S Antibody [Unconjugated]

 (19 Publications)
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Cathepsin S was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human Cathepsin S Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1183) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
Cathepsin S was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human Cathepsin S Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1183) at 15 µg/mL overnight ...read more
CTSS attenuates EGF-mediated EGFR degradation.(a) OEC-M1 and MDA-MB-231 cells were pretreated with 20 μM 6r or ZFL for 1 h and subsequently incubated with 100 ng/mL EGF for an additional 2 h. The total cell lysates ...read more
Genetic Strategies: CTSS inhibition does not impair lysosomal activities.(a) OEC-M1 cells were pretreated with vesicle or 100 nM BAF for 1 h and then incubated with 20 μM 6r for 1 h further. Lysosomal ...read more
Myc induces cathepsin L expression in beta-cells of pancreatic Islets.(A) Immunohistochemical analyses for CTS B, C, L or S expression (all in red) in combination with staining for the pan-leukocyte marker CD45 (green) ...read more
Myc induces cathepsin L expression in beta-cells of pancreatic Islets.(A) Immunohistochemical analyses for CTS B, C, L or S expression (all in red) in combination with staining for the pan-leukocyte marker CD45 (green) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF1183
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1183-SP
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Cathepsin S Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin S
Gln17-Ile331
Accession # P25774
Specificity
Detects human Cathepsin S in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human Cathepsin A, B, C, D, E, L, V, and X/Z/P is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSS
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Neutralization 0.2-1.8 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human Cathepsin S Antibody (Catalog # AF1183)
ELISA Detection: Human Cathepsin S Biotinylated Antibody (Catalog # BAF1183)
Standard: Recombinant Human Cathepsin S (Catalog # 1183-CY)
Publications
Read Publications using
AF1183 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin S Antibody [Unconjugated]

  • Cathepsin S
  • CTSS
  • EC 3.4.22
  • EC 3.4.22.27
  • FLJ50259
  • MGC3886

Background

Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1-16), a pro region (residues 17-114), and the mature enzyme (residues 115-331) (3-5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages, and other phagocytic cells.

  1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104 - 1107, Academic Press, San Diego.
  2. Turk, V. et al. (2001) EMBO J. 20:4629.
  3. Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
  4. Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
  5. Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin S Antibody (AF1183)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 5 applications: Flow Cytometry, IHC, IHC-P, Luminex Development, Western Blot.


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Flow Cytometry
(1)
IHC
(1)
IHC-P
(3)
Luminex Development
(1)
Western Blot
(9)
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Human
(10)
Mouse
(4)
Chicken
(1)
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using AF1183 Applications Species
Wang S, Luke CJ, Pak SC et al. SERPINB3 (SCCA1) inhibits cathepsin L and lysoptosis, protecting cervical cancer cells from chemoradiation Communications Biology 2022-01-12 [PMID: 35022555]
Wang S, Luke CJ, Pak SC et al. SERPINB3 (SCCA1) inhibits cathepsin L and lysoptosis, protecting cervical cancer cells from chemoradiation Communications Biology 1/12/2022 [PMID: 35022555]
D Bararia, JA Hildebrand, S Stolz, S Haebe, S Alig, CP Trevisani, F Osorio-Bar, MD Bartoschek, M Mentz, A Pastore, E Gaitzsch, M Heide, V Jurinovic, K Rautter, J Gunawardan, MB Sabdia, M Szczepanow, J Richter, W Klapper, A Louissaint, C Ludwig, S Bultmann, H Leonhardt, S Eustermann, KP Hopfner, W Hiddemann, M von Bergwe, C Steidl, R Kridel, JWD Tobin, MK Gandhi, DM Weinstock, M Schmidt-Su, MB Sárosi, M Rudelius, V Passerini, J Mautner, O Weigert Cathepsin S Alterations Induce a Tumor-Promoting Immune Microenvironment in Follicular Lymphoma Cell Rep, 2020-04-23;31(5):107522. 2020-04-23 [PMID: 32330423] (Western Blot, Human) Western Blot Human
Tina Zavašnik-Bergant, Robert Vidmar, Andreja Sekirnik, Marko Fonović, Jiří Salát, Lenka Grunclová et al. Salivary Tick Cystatin OmC2 Targets Lysosomal Cathepsins S and C in Human Dendritic Cells Frontiers in Cellular and Infection Microbiology 6/30/2017 [PMID: 28713775]
The Unusual Resistance of Avian Defensin AvBD7 to Proteolytic Enzymes Preserves Its Antibacterial Activity PLoS ONE, 2016-08-25;11(8):e0161573. 2016-08-25 [PMID: 27561012] (Western Blot, Chicken) Western Blot Chicken
Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors Sci Rep, 2016-07-08;6(0):29256. 2016-07-08 [PMID: 27387133] (Western Blot, Human) Western Blot Human
Evaluating the diagnostic and prognostic value of circulating cathepsin S in gastric cancer Oncotarget, 2016-05-10;7(19):28124-38. 2016-05-10 [PMID: 27058412] (IHC-P, Human) IHC-P Human
Y Hamon, M Legowska, V Hervé, S Dallet-Cho, S Marchand-A, L Vanderlynd, M Demonte, R Williams, CJ Scott, M Si-Tahar, N Heuzé-Vour, G Lalmanach, DE Jenne, A Lesner, F Gauthier, B Korkmaz Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases J. Biol. Chem., 2016-02-16;291(16):8486-99. 2016-02-16 [PMID: 26884336] (Western Blot, Human) Western Blot Human
Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer. PLoS ONE, 2015-04-30;10(4):e0120348. 2015-04-30 [PMID: 25927437] (IHC, Mouse) IHC Mouse
Louisa Ben-Aderet, Emmanuelle Merquiol, Duha Fahham, Ashok Kumar, Eli Reich, Yael Ben-Nun et al. Detecting cathepsin activity in human osteoarthritis via activity-based probes Arthritis Research & Therapy 12/1/2015 [PMID: 25889265]
Show All 19 Publications.

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Bioinformatics

Gene Symbol CTSS
Uniprot