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Catalase Antibody (OTI1B8)

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Western Blot: Catalase Antibody (1B8) [NBP2-00492] Analysis of extracts (35ug) from 9 different cell lines by using anti-Catalase monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Catalase Antibody (OTI1B8) [NBP2-00492] - Mouse hepatocytes stained with catalase antibody. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human lung tissue using anti-Catalase mouse monoclonal antibody.
Flow Cytometry: Catalase Antibody (1B8) [NBP2-00492] - Analysis of Jurkat cells, using anti-Catalase antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: Catalase Antibody (1B8) [NBP2-00492] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Catalase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: Catalase Antibody (1B8) [NBP2-00492] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Catalase.
Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Catalase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Catalase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human endometrium tissue using anti-Catalase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human liver tissue using anti-Catalase mouse monoclonal antibody.
Flow Cytometry: Catalase Antibody (1B8) [NBP2-00492] - Analysis of Hela cells, using anti-Catalase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Catalase Antibody (OTI1B8) Summary

Immunogen
Full length human recombinant protein of human CAT (NP_111743) produced in HEK293T cell.
Marker
Peroxisome Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CAT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
59.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-00492 in the following applications:

Publications
Read Publication using
NBP2-00492 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA, 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Catalase Antibody (OTI1B8)

  • Cas1
  • CAT
  • Catalase
  • Cs-1
  • EC 1.11.1.6
  • MGC138422
  • MGC138424

Background

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Catalase Antibody (NBP2-00492)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Immunohistochemistry.


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Review for Catalase Antibody (NBP2-00492) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-00492:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
ICC Mouse 11/05/2021
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Summary

ApplicationImmunocytochemistry
Sample TestedHepatocytes
SpeciesMouse
LotOTI1B8

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Video Protocols

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FAQs for Catalase Antibody (NBP2-00492) (0)

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Recent Reviews

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Verified Customer
11/05/2021
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol CAT
Entrez