Home » Calreticulin » Calreticulin Antibodies » Calreticulin Antibody (681233) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

Calreticulin Antibody (681233) [Unconjugated]

 (3 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Calreticulin Monoclonal Antibody (Catalog # MAB38981) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Calreticulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and NRK rat normal kidney cell line. PVDF membrane was ...read more
Calreticulin was detected in formalin fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Calreticulin Monoclonal Antibody (Catalog # MAB38981) at 15 µg/mL overnight at 4 °C. Before incubation ...read more
HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Calreticulin Monoclonal Antibody (Catalog # MAB38981, filled histogram) or isotype control antibody (Catalog # MAB0041, open ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, Flow, KO
Clone
681233
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC38981U-100UG
Alexa Fluor 405
IC38981V-100UG
Alexa Fluor 488
IC38981G-100UG
Alexa Fluor 532
FAB38981X
Alexa Fluor 594
IC38981T-100UG
Alexa Fluor 647
IC38981R-100UG
Alexa Fluor 700
IC38981N-100UG
Alexa Fluor 750
IC38981S-100UG
Allophycocyanin
FAB38981A
Biotin
FAB38981B
CoraFluor 1
FAB38981CL1
DyLight 350
FAB38981D
DyLight 405
FAB38981E
DyLight 488
FAB38981K
DyLight 550
FAB38981L
DyLight 594
FAB38981M
DyLight 650
FAB38981W
DyLight 680
FAB38981Y
DyLight 755
FAB38981Z
FITC
FAB38981F
HRP
FAB38981H
Janelia Fluor 525
FAB38981JF525
Janelia Fluor 549
FAB38981I
Janelia Fluor 646
FAB38981J
Janelia Fluor 669
FAB38981JF669
PE
FAB38981P
PerCP
FAB38981C
mFluor Violet 450 SE
FAB38981MFV450
mFluor Violet 500 SE
FAB38981MFV500
mFluor Violet 610 SE
FAB38981MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB38981-SP
Carrier Free
IC38981V-100UG
Carrier Free
IC38981U-100UG
Carrier Free
IC38981T-100UG
Carrier Free
IC38981S-100UG
Carrier Free
IC38981R-100UG
Carrier Free
IC38981N-100UG
Carrier Free
IC38981G-100UG
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Calreticulin Antibody (681233) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Calreticulin
Glu18-Leu417
Accession # P27797
Specificity
Detects human Calreticulin in ELISA. Detects human, mouse and rat Calreticulin in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CALR
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB38981 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calreticulin Antibody (681233) [Unconjugated]

  • Autoantigen Ro
  • CALR
  • Calregulin
  • Calreticulin
  • cC1qR
  • CRP55
  • CRT
  • Endoplasmic reticulum resident protein 60
  • epididymis secretory sperm binding protein Li 99n
  • ERp60
  • FLJ26680
  • grp60
  • HACBP
  • HEL-S-99n
  • RO
  • Sicca syndrome antigen A (autoantigen Ro; calreticulin)
  • SSA
  • Vasostatin

Background

Human Calreticulin is a 55‑60 kDa, 400 amino acid, variably glycosylated intra- and extracellular Ca++-binding lectin that is ubiquitously expressed. It consists of three domains: a 180 aa N-terminal globular region, a 111 aa P-, or proline rich domain, and a 109 aa C-terminus. The 180 aa N-terminus (aa 18‑197) is termed Vasostatin. It is unclear if it is ever generated naturally via proteolytic processing. Vasostatin domain has many functions. It binds to RNA (aa 18‑27), has autocatalytic phosphorylase activity (aa 77‑197), binds to a KxFFKR motif on steroid hormone receptors, and serves as a lectin-type chaperone for ER-localized molecules. It also shows antiangiogenic activity, presumably by binding to laminin carbohydrates and blocking endothelial cell adhesion and proliferation. Human Calreticulin is 94% aa identical to mouse and rat Calreticulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

SSB Antibody
(OTI2C8)
NBP1-48002
Western Blot SSB Antibody (OTI2C8)
Immunohistochemistry-Paraffin SSB Antibody (OTI2C8)
Immunohistochemistry-Paraffin SSB Antibody (OTI2C8)
Species: Ca, Hu, Pm, Pm
Applications: ICC/IF, IHC, IHC-P, IP, WB
TROVE2 Antibody
NBP1-86998
Immunohistochemistry-Paraffin TROVE2 Antibody
Western Blot TROVE2 Antibody
Western Blot TROVE2 Antibody
(1 Review)
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, IP, WB
TRIM21/SSA1
Antibody
NBP1-33548
Immunocytochemistry/ Immunofluorescence TRIM21/SSA1 Antibody
Immunohistochemistry-Paraffin TRIM21/SSA1 Antibody
Western Blot TRIM21/SSA1 Antibody
(4 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, KD, WB
Calnexin Antibody
- BSA Free
NB100-1965
Western Blot Calnexin Antibody - BSA Free
Immunohistochemistry Calnexin Antibody - BSA Free
Immunohistochemistry Calnexin Antibody - BSA Free
(3 Reviews)
(46 Publications)
Species: Av, Bv, Ch, Dr, Gp, Hu, Mu, Po, Rb, Rt, Sh, Xp, Ze
Applications: Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
PRRT2 Antibody -
BSA Free
NBP2-82026
Western Blot PRRT2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence PRRT2 Antibody - BSA Free
Immunohistochemistry PRRT2 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
GRP78/HSPA5
Antibody - BSA Free
NBP1-06274
Flow Cytometry GRP78/HSPA5 Antibody - BSA Free
Flow Cytometry GRP78/HSPA5 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence GRP78/HSPA5 Antibody - BSA Free
(2 Reviews)
(44 Publications)
Species: Ce, Ch, Dr, Hu, Mu, Rt, Sh, Ze
Applications: Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, Simple Western, WB
ERp57/PDIA3
Antibody
NBP1-84796
Western Blot ERp57/PDIA3 Antibody
Immunohistochemistry-Paraffin ERp57/PDIA3 Antibody
Immunohistochemistry-Paraffin ERp57/PDIA3 Antibody
(4 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
POMT1 Antibody
NBP3-12487
Immunohistochemistry-Paraffin POMT1 Antibody
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
gp96/HSP90B1/GRP94
Antibody (9G10)
NB300-619
Immunocytochemistry/ Immunofluorescence gp96/HSP90B1/GRP94 Antibody (9G10)
Immunocytochemistry/ Immunofluorescence gp96/HSP90B1/GRP94 Antibody (9G10)
Immunocytochemistry/ Immunofluorescence gp96/HSP90B1/GRP94 Antibody (9G10)
(2 Reviews)
(5 Publications)
Species: Bv, Ch, Ha, Hu, Mu, Rt
Applications: ICC, ICC/IF, IHC, IHC-P, IP, WB
RNPC3 Antibody -
BSA Free
NBP2-93867
Western Blot RNPC3 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: WB
CD77 Synthase
Antibody
NBP1-62583
Western Blot CD77 Synthase Antibody
(2 Publications)
Species: Hu
Applications: WB
BMP-3b/GDF-10
Antibody [Unconjugate
AF1543
Immunohistochemistry BMP-3b/GDF-10 Antibody [Unconjugated]
(1 Publication)
Species: Hu
Applications: IHC, WB
MID1 Antibody
NBP1-26612
Western Blot MID1 Antibody
(2 Publications)
Species: Hu
Applications: IP (-), WB
Recombinant Human
PRH2 GST (N-Term) Pro ...
H00005555-P01
SDS-PAGE Recombinant Human PRH2 GST (N-Term) Protein
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
ZAK Antibody (3G5)
H00051776-M03
Western Blot ZAK Antibody (3G5)
Immunocytochemistry/ Immunofluorescence ZAK Antibody (3G5)
Immunohistochemistry-Paraffin ZAK Antibody (3G5)
(3 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(685 Publications)
Species: Hu
Applications: ELISA
SEC14L2 Antibody
(OTI4H2)
NBP2-01346
Flow Cytometry SEC14L2 Antibody (OTI4H2)
Western Blot SEC14L2 Antibody (OTI4H2)
Immunohistochemistry-Paraffin SEC14L2 Antibody (OTI4H2)
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB

Publications for Calreticulin Antibody (MAB38981)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Flow Cytometry, ICC.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species

Reviews for Calreticulin Antibody (MAB38981) (0)

There are no reviews for Calreticulin Antibody (MAB38981). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Calreticulin Antibody (MAB38981) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Calreticulin Products

Blogs on Calreticulin.

Check out the latest blog posts on Calreticulin.
Calreticulin - ER chaperone involved in calcium homeostasis and protein quality control
Calreticulin is a calcium-dependent ER chaperone, involved in protein folding, maturation, and cellular localization. Calreticulin is a highly conserved 48 kDa protein encoded by the CALR gene. Calreticulin and its homolog calnexin regulate the fo...  Read full blog post.
Calnexin - an ER chaperone that folds the cell's glycoproteins
Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded...  Read full blog post.
Calreticulin: a Multiprocess Calcium Buffering Chaperone
Calreticulin is a Calcium binding chaperone that has multiple functions both inside and outside the endoplasmic reticulum. Calreticulin is involved in the quality control of newly synthesized proteins and glycoproteins, interacting with various other ...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Calreticulin Antibody (681233) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol CALR
Uniprot