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Calbindin D-28K Antibody (CALB1/2782)

Images

 
Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (CALB1/2782) [NBP3-07208] - Formalin-fixed, paraffin-embedded human Kidney stained with Calbindin D-28K Mouse Monoclonal Antibody (CALB1/2782).
Protein Array: Calbindin D-28K Antibody (CALB1/2782) [NBP3-07208] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Calbindin D-28K Mouse Monoclonal Antibody (CALB1/2782).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC, MA
Clone
CALB1/2782
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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Calbindin D-28K Antibody (CALB1/2782) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08954)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant fragment (around aa 7-96) of human Calbindin D-28K protein (exact sequence is proprietary) (Uniprot: P05937)
Localization
Nuclear and Cytoplasmic
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CALB1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Calbindin D-28K Antibody (CALB1/2782)

  • CAB27
  • CALB
  • calbindin 1, (28kD)
  • calbindin 1, 28kDa
  • Calbindin D28
  • calbindin
  • D-28K
  • RTVL-H protein
  • Vitamin D-dependent calcium-binding protein, avian-type

Background

Calbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. Calbindin antibody is often used as a marker for cerebellum Purkinje cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Calbindin D-28K Antibody (NBP3-07208). (Showing 1 - 1 of 1 FAQ).

  1. I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?
    • This link will take you to all of our alpha7 nicotinic cholingergic receptor antibodies currently available for purchase. Please see the following link for our Calbinin D28K antibodies reactive against mouse and human. We currently do not have any Parvalbumin antibodies that have been tested in mouse. I would like to introduce you to our Innovators Reward Program. Try our primary antibodies in an untested species or application, without the financial risk of failure. The pricing and availability of our products depends on your country.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CALB1
Uniprot