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C5L2/GPR77 Antibody (650918) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with (A) C5L2/GPR77 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human C5L2/GPR77 Monoclonal Antibody (Catalog # MAB10254) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with (A) C5L2/GPR77 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human C5L2/GPR77 Monoclonal Antibody (Catalog # MAB10254) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
650918
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB10254U-100UG
Alexa Fluor 405
FAB10254V-100UG
Alexa Fluor 488
FAB10254G-100UG
Alexa Fluor 532
FAB10254X
Alexa Fluor 594
FAB10254T-100UG
Alexa Fluor 647
FAB10254R-100UG
Alexa Fluor 700
FAB10254N-100UG
Alexa Fluor 750
FAB10254S-100UG
Allophycocyanin
FAB10254A
Allophycocyanin/Cy7
FAB10254APCCY7
Biotin
FAB10254B
CoraFluor 1
FAB10254CL1
DyLight 350
FAB10254D
DyLight 405
FAB10254E
DyLight 488
FAB10254K
DyLight 550
FAB10254L
DyLight 594
FAB10254M
DyLight 650
FAB10254W
DyLight 680
FAB10254Y
DyLight 755
FAB10254Z
FITC
FAB10254F
HRP
FAB10254H
Janelia Fluor 525
FAB10254JF525
Janelia Fluor 549
FAB10254I
Janelia Fluor 646
FAB10254J
Janelia Fluor 669
FAB10254JF669
PE
FAB10254P
PE/Atto594
FAB10254PEATT594
PE/Cy5.5
FAB10254PECY55
PE/Cy7
FAB10254PECY7
PerCP
FAB10254C
mFluor Violet 450 SE
FAB10254MFV450
mFluor Violet 500 SE
FAB10254MFV500
mFluor Violet 610 SE
FAB10254MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB10254-SP
Carrier Free
FAB10254V-100UG
Carrier Free
FAB10254U-100UG
Carrier Free
FAB10254T-100UG
Carrier Free
FAB10254S-100UG
Carrier Free
FAB10254R-100UG
Carrier Free
FAB10254N-100UG
Carrier Free
FAB10254G-100UG
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C5L2/GPR77 Antibody (650918) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human C5L2/GPR77
Met1-Val337
Accession # Q9P296
Specificity
Detects human C5L2/GPR77 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publication using
MAB10254 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for C5L2/GPR77 Antibody (650918) [Unconjugated]

  • C5a anaphylatoxin chemotactic receptor C5L2
  • C5L2
  • C5L2G-protein coupled receptor 77
  • G protein-coupled receptor 77
  • G protein-coupled receptor C5L2
  • GPF77
  • GPR77

Background

C5a anaphylatoxin chemotactic receptor C5a2, also known as C5L2 or GPR77, is a G protein-coupled receptor that in humans is encoded by the C5AR2. C5a2 is the receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides generated during the complement cascade that participate in host defense.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for C5L2/GPR77 Antibody (MAB10254)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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