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ASPM Antibody

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Immunocytochemistry/Immunofluorescence: ASPM Antibody [NB100-2278] - Samples: NBF-fixed asynchronous HeLa cells . Antibody: Affinity purified rabbit anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit IgG (h&l) FITC used at a dilution of 1:100.
Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278] - Samples: NBF-fixed asynchronous HeLa cells . Antibody: Affinity purified rabbit anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278] - Detection of Human ASPM in NBF-fixed asynchronous HeLa cells with anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit IgG (h&l) FITC ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.25 mg/ml

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ASPM Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 3425 and the C-terminus (residue 3477) of human ASP (Abnormal Spindle)-like, Microcephaly Associated using the numbering given in entry NP_060606.2 (GeneID 259266).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ASPM
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin
Application Notes
For ICC/IF: Permeabilization with Triton-X 100 is recommended. Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.Likely to work with frozen sections. IHC was reported in scientific literature (PMID: 22350525). Use in IHC-P reported in scientific literature (PMID: 30092011).
Publications
Read Publications using
NB100-2278 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22350525).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.25 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASPM Antibody

  • asp (abnormal spindle) homolog, microcephaly associated (Drosophila)
  • Asp homolog
  • ASP
  • Calmbp1
  • FLJ43117
  • MCPH5DKFZp686N06184
  • microcephaly associated (Drosophila)
  • microcephaly, primary autosomal recessive 5

Background

The ASPM gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts (Bond et al., 2002). The mouse gene Aspm is expressed specifically in the primary sites of prenatal cerebral cortical neurogenesis.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASPM Antibody (NB100-2278)(10)

We have publications tested in 5 confirmed species: Human, Mouse, Bovine, Porcine, Sheep.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-P, Immunocytochemistry/ Immunofluorescence.


Filter By Application
ICC/IF
(4)
IF/IHC
(1)
IHC-P
(1)
Immunocytochemistry/ Immunofluorescence
(1)
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Human
(2)
Mouse
(3)
Bovine
(1)
Porcine
(1)
Sheep
(1)
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Showing Publications 1 - 10 of 10.
Publications using NB100-2278 Applications Species
Hei-Yong G Lo, Chad G Pearson, J Matthew Taliaferro Differential subcellular localization of ASPM RNA and protein microPublication Biology 2024-01-24 [PMID: 38344067]
Li Y, Liu Y, Gao Z et al. Single-cell transcriptomes of mouse bladder urothelium uncover novel cell type markers and urothelial differentiation characteristics Cell Proliferation 2021-04-01 [PMID: 33538002] (Immunocytochemistry/ Immunofluorescence) Immunocytochemistry/ Immunofluorescence
Chinen T, Yamamoto S, Takeda Y et al. NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells EMBO J. 2019-11-29 [PMID: 31782546] (ICC/IF) ICC/IF
Gemble S, Simon A, Pennetier C et al. Centromere Dysfunction Compromises Mitotic Spindle Pole Integrity Curr. Biol. 2019-09-23 [PMID: 31495582] (Human) Human
So C, Seres K. B, et al. A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Science 2019-06-28 [PMID: 31249032] (ICC/IF, Mouse, Sheep, Porcine, Bovine) ICC/IF Mouse, Sheep, Porcine, Bovine
Raman P, Maddipati R, Lim KH et al. Pancreatic cancer survival analysis defines a signature that predicts outcome PLoS ONE 2018-08-09 [PMID: 30092011] (IHC-P, Human) IHC-P Human
Jiang K, Rezabkova L, Hua S et al. Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex. Nat. Cell Biol. 2017-05-01 [PMID: 28436967] (ICC/IF) ICC/IF
Sgourdou P, Mishra-Gorur K, Saotome I et al. Disruptions in asymmetric centrosome inheritance and WDR62-Aurora kinase B interactions in primary microcephaly. Sci Rep. 2017-03-08 [PMID: 28272472] (ICC/IF, Mouse) ICC/IF Mouse
Bainer RO, Veneris JT, Yamada SD et al. Time-dependent transcriptional profiling links gene expression to mitogen-activated protein kinase kinase 4 (MKK4)-mediated suppression of omental metastatic colonization. Clin Exp Metastasis 2012-06-01 [PMID: 22350525] (IF/IHC, Mouse) IF/IHC Mouse
Griffith E, Walker S, Martin CA et al. Mutations in pericentrin cause Seckel syndrome with defective ATR-dependent DNA damage signaling. Nat Genet 2008-02-01 [PMID: 18157127]

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Array NB100-2278

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Bioinformatics

Gene Symbol ASPM
Entrez
Uniprot