Home » Thyroglobulin » Thyroglobulin Antibodies » Thyroglobulin Antibody (904802) [Unconjugated]

Thyroglobulin Antibody (904802) [Unconjugated]

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Western blot shows lysates of human thyroid tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Thyroglobulin Monoclonal Antibody (Catalog # MAB8306) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Thyroglobulin at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human thyroid tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Thyroglobulin Monoclonal Antibody (Catalog # MAB8306) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more
Thyroglobulin was detected in immersion fixed paraffin-embedded sections of human thyroid cancer tissue using Mouse Anti-Human Thyroglobulin Monoclonal Antibody (Catalog # MAB8306) at 15 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
904802
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB8306-SP
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Thyroglobulin Antibody (904802) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Thyroglobulin
Met1-Lys2768
Accession # P01266
Specificity
Detects human Thyroglobulin in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TG
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB8306 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thyroglobulin Antibody (904802) [Unconjugated]

  • AITD3
  • AITD3TGN
  • cog
  • TDH3
  • Tg
  • TGN
  • Thyroglobulin

Background

Human Thyroglobulin (Tg) gene is localized at position 8q242-8q243. The gene covers a genome area of at least 300 000 bp and contains at least 37 exons. Thyroglobulin (Tg) is a dimeric 660 kDa glycoprotein produced by follicular cells (thyrocytes) in thyroid gland and stored in the follicular colloid of the gland. Tg is heterogenic due to post-translational modifications, such as iodination, glycosylation, sulfatation, phosphorylation and it is highly sensitivity to proteolysis.  Acting as a substrate, Tg is used for the synthesis of thyroid hormones thyroxine (T4) and triiodothyronine (T3).  Tg level can serve as a tumor marker of papillary and follicular thyroid cancer and elevated levels of anti-Tg antibodies in blood can be indicative of Graves disease. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Thyroglobulin Antibody (MAB8306) (0)

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Review for Thyroglobulin Antibody (MAB8306) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: horse.

Reviews using MAB8306:
Filter by Applications
IHC-P
(1)
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horse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Thyroglobulin MAB8306
Enlarge 		4
reviewed by:
Christof Bertram
 IHC-P horse 01/26/2017
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedthyroid gland and thyroid follicular tumor
Specieshorse
LotCISD011611A

Comments

CommentsApplication: Immunohistochemistry

Species: Horse
Dilution: 1:100
Pretreatment for antigen retrieval: microwave heating with citrate buffer for 12 minutes at 600 watts

Results: positive staining of follicular epithelium

Product General Protocols

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FAQs for Thyroglobulin Antibody (MAB8306) (0)

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Secondary Antibodies

 

Isotype Controls

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TTF1 / NKX2.1 - An essential regulator of lung development with implications in cancer diagnostics
Thyroid transcription factor 1 (TTF-1), also known as NKX2.1, is a conserved master regulatory transcription factor involved in the development of the lung, brain, and thyroid (1). In the lung TTF-1 positively regulates the expression of several lu...  Read full blog post.
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Recent Reviews

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Immunohistochemistry-Paraffin Thyroglobulin MAB8306
Enlarge
 Christof Bertram
01/26/2017
Application: IHC-P
Species: horse
read this review

Bioinformatics

Gene Symbol TG
Uniprot