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C5L2/GPR77 Antibody (650918) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
650918
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB10254U-100UG
Alexa Fluor 405
FAB10254V-100UG
Alexa Fluor 488
FAB10254G-100UG
Alexa Fluor 532
FAB10254X
Alexa Fluor 594
FAB10254T-100UG
Alexa Fluor 647
FAB10254R-100UG
Alexa Fluor 700
FAB10254N-100UG
Alexa Fluor 750
FAB10254S-100UG
Allophycocyanin
FAB10254A
Allophycocyanin/Cy7
FAB10254APCCY7
Biotin
FAB10254B
DyLight 350
FAB10254D
DyLight 405
FAB10254E
DyLight 488
FAB10254K
DyLight 550
FAB10254L
DyLight 594
FAB10254M
DyLight 650
FAB10254W
DyLight 680
FAB10254Y
DyLight 755
FAB10254Z
FITC
FAB10254F
HRP
FAB10254H
Janelia Fluor 525
FAB10254JF525
Janelia Fluor 549
FAB10254I
Janelia Fluor 646
FAB10254J
Janelia Fluor 669
FAB10254JF669
PE
FAB10254P
PE/Atto594
FAB10254PEATT594
PE/Cy5.5
FAB10254PECY55
PE/Cy7
FAB10254PECY7
PerCP
FAB10254C
Unconjugated
MAB10254-100
mFluor Violet 450 SE
FAB10254MFV450
mFluor Violet 500 SE
FAB10254MFV500
mFluor Violet 610 SE
FAB10254MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB10254-100
Carrier Free
MAB10254-100
Carrier Free
MAB10254-SP
Carrier Free
FAB10254V-100UG
Carrier Free
FAB10254U-100UG
Carrier Free
FAB10254T-100UG
Carrier Free
FAB10254S-100UG
Carrier Free
FAB10254R-100UG
Carrier Free
FAB10254N-100UG
Carrier Free
FAB10254G-100UG
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C5L2/GPR77 Antibody (650918) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
NS0 mouse myeloma cell line transfected with human C5L2/GPR77
Met1-Val337
Accession # Q9P296
Specificity
Detects human C5L2/GPR77 in direct ELISAs.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for C5L2/GPR77 Antibody (650918) [CoraFluor™ 1]

  • C5a anaphylatoxin chemotactic receptor C5L2
  • C5L2
  • C5L2G-protein coupled receptor 77
  • G protein-coupled receptor 77
  • G protein-coupled receptor C5L2
  • GPF77
  • GPR77

Background

Official Gene Symbol: GPR77 Gen Bank Accession Number: NP_060955 Gene ID: 27202 (human) Gene Map Locus: 19q13.33 (human) C5L2 is 337A.A orphan member that belongs to anaphylatoxin receptor family. It is a seven transmembrane G-protein-coupled receptor consisting of an N-terminal N-glycosylation site, several conserved cysteines and several potential C-terminal phosphorylation sites. It binds to C5a and C5a-des-Arg and serves as a highly regulated scavenger receptor with a negative modulatory influence on C5aR-mediated inflammation. Northern Blot analysis suggests it is co- expressed along with C5aR in different tissues and cells including neutrophils/macrophages, mast cells, dendritic cells, brain, lung, heart, kidney, liver, ovary and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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