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Brachyury Antibody [Phycoerythrin]

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D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was stained with Goat Anti-Human/Mouse Brachyury PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085P, filled histogram) or isotype control antibody (IC108P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was stained with Goat Anti-Human/Mouse Brachyury PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085P, filled ...read more
NCI-H460 cells were stained with Goat Anti-Human/Mouse Brachyury PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085P, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 488
IC2085G
Allophycocyanin
IC2085A
Biotin
BAF2085
NL557
NL2085R
Unconjugated
AF2085
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF2085
Carrier Free
AF2085
Carrier Free
IC2085A
Carrier Free
IC2085G
Carrier Free
NL2085R
Carrier Free
AF2085-SP
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Brachyury Antibody [Phycoerythrin] Summary

Immunogen
E. coli-derived recombinant human Brachyury
Ser2-Glu202
Accession # O15178
Specificity
Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
T
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC2085P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brachyury Antibody [Phycoerythrin]

  • brachyury protein
  • Brachyury
  • MGC104817
  • Protein T
  • SAVA
  • T brachyury (mouse) homolog
  • T brachyury homolog
  • T Brachyury Transcription Factor
  • T, brachyury homolog (mouse)
  • T-Box Transcription Factor T
  • TBXT
  • TF
  • TFT

Background

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Brachyury Antibody (IC2085P)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 3 applications: Flow Cytometry, ICC, Western Blot.


Filter By Application
Flow Cytometry
(4)
ICC
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Porcine
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using IC2085P Applications Species
Motoyasu Hosokawa, Ryuta Mikawa, Atsuko Hagiwara, Yukiko Okuno, Tomonari Awaya, Yuki Yamamoto et al. Cryptotanshinone is a candidate therapeutic agent for interstitial lung disease associated with a BRICHOS-domain mutation of SFTPC iScience 2023-10-20 [PMID: 37701577]
Y Lan, KM Banks, H Pan, N Verma, GR Dixon, T Zhou, B Ding, O Elemento, S Chen, D Huangfu, T Evans Stage-specific regulation of DNA methylation by TET enzymes during human cardiac differentiation Cell Reports, 2021-12-07;37(10):110095. 2021-12-07 [PMID: 34879277] (Flow Cytometry, ICC, Western Blot, Human) Flow Cytometry, ICC, Western Blot Human
L Jin, C Chang, KM Pawlik, A Datta, LM Johnson, T Vu, JL Napoli, PK Datta STRAP Deficiency Impairs Mouse Embryonic Stem Cells Lineage Commitment Through CYP26A1-Mediated Retinoic Acid Homeostasis Stem Cells, 2018-07-17;0(0):. 2018-07-17 [PMID: 29781215] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Annabelle Congras Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells Sci Rep, 2016-06-01;6(0):27059. 2016-06-01 [PMID: 27245508] (Flow Cytometry, Porcine) Flow Cytometry Porcine
Yijie Geng, Bradley Feng Mesendogen, a novel inhibitor of TRPM6, promotes mesoderm and definitive endoderm differentiation of human embryonic stem cells through alteration of magnesium homeostasis Heliyon 2015-12-05 [PMID: 26705539]
Vijayaragavan K, Szabo E, Bosse M, Ramos-Mejia V, Moon RT, Bhatia M Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells. Cell Stem Cell, 2009-03-06;4(3):248-62. 2009-03-06 [PMID: 19265664] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol T
Uniprot