![Western Blot: BP1 Overexpression Lysate (Adult Normal) [NBL1-09920] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BP1.](http://images.novusbio.com/fullsize/BP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09920-img0002.jpg "Western Blot: BP1 Overexpression Lysate (Adult Normal) [NBL1-09920] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BP1.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Description | BP1 Transient Overexpression LysateExpression Host: HEK293T Plasmid: RC223036 Accession#: NM_001934 Protein Tag: C-MYC/DDK You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT. |
| Gene | DLX4 |
| Dilutions |
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| Application Notes | This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. |
| Theoretical MW | 18.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RIPA buffer |
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BP1 Antibodies, Beta Globin and Breast Cancer: Today's post is brought to you by the letter 'B' The transcription factor beta protein 1 (BP1) is a member of the homeobox gene family and the distal-less subfamily. Expression of BP1 is highly tissue-specific and developmentally restricted. Among different human tissues, BP1 is found to be highly e... Read full blog post. |
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| Gene Symbol | DLX4 |
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![Western Blot: BP1 Antibody [NB100-481] - Effect of BP1 on TNFalpha-induced cell death. Western blot analysis of Beta Protein 1 (BP1) protein expression in MCF7/EV cell lines and MCF7/BP1 cell lines. Image collected and cropped by CiteAb from the following publication (https://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr1766), licensed under a CC-BY license.](https://images.novusbio.com/images/BP1-Antibody-Western-Blot-NB100-481-img0007.jpg "Western Blot: BP1 Antibody [NB100-481] - Effect of BP1 on TNFalpha-induced cell death. Western blot analysis of Beta Protein 1 (BP1) protein expression in MCF7/EV cell lines and MCF7/BP1 cell lines. Image collected and cropped by CiteAb from the following publication (https://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr1766), licensed under a CC-BY license.")