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BMP-7 Antibody (6E5D12) - BSA Free

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Western Blot: BMP-7 Antibody (6E5D12) [NBP2-52425] - Analysis using BMP7 mouse mAb against Raw264.7 (1), A549 (2), Jurkat (3), PC-3 (4), HEK293 (5), Jurkat (6), NIH/3T3 (7), and Hela (8) cell lysate.
Western Blot: BMP-7 Antibody (6E5D12) [NBP2-52425] - Analysis using BMP7 mouse mAb against Raw264.7 (1), A549 (2), Jurkat (3), PC-3 (4), HEK293 (5), Jurkat (6), NIH/3T3 (7), and Hela (8) cell lysate.
Immunohistochemistry-Paraffin: BMP-7 Antibody (6E5D12) [NBP2-52425] - Esophageal cancer tissues using BMP7 mouse mAb with DAB staining.
Flow Cytometry: BMP-7 Antibody (6E5D12) [NBP2-52425] - Analysis of HEK293 cells using BMP7 mouse mAb (green) and negative control (red).
Western Blot: BMP-7 Antibody (6E5D12) [NBP2-52425] - Analysis using BMP7 mAb against HEK293 (1) and BMP7 (AA: 239-431)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: BMP-7 Antibody (6E5D12) [NBP2-52425] - Analysis using BMP7 mAb against human BMP7 recombinant protein. (Expected MW is 47.7 kDa)
ELISA: BMP-7 Antibody (6E5D12) [NBP2-52425] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Product Details

Summary
Reactivity Hu, Mu, V-ViSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
6E5D12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-52425-0.025mg
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BMP-7 Antibody (6E5D12) - BSA Free Summary

Immunogen
Purified recombinant fragment of human BMP-7 (AA: 239-431) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BMP7
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
49.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-52425 in the following applications:

  • WB
    1 publication

Reactivity Notes

Viral reactivity reported in scientific literature (PMID: 30850350). Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for BMP-7 Antibody (6E5D12) - BSA Free

  • BMP7
  • BMP-7
  • bone morphogenetic protein 7
  • Eptotermin alfa
  • OP-1
  • OP-1OP1
  • Osteogenic protein 1

Background

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-52425
BMP-7 Antibody (6E5D12) - BSA Free
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Publications for BMP-7 Antibody (NBP2-52425)(1)

We have publications tested in 1 confirmed species: Viral.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol BMP7