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Recombinant Human BMP-2 Protein

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SDS-Page: Human BMP-2 Protein [NBP2-34928] - The Recombinant Human BMP-2 homodimeric protein is resolved by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

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Recombinant Human BMP-2 Protein Summary

Description
A homodimeric protein consisting of two 115 amino acid non-glycosylated polypeptide chains corresponding to BMP-2 Source: E. coli

Uniprot ID: P12643

Amino Acid Sequence: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR

Details of Functionality
BMP2 protein is fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/ml, corresponding to a specific activity of > 5.0 x 10^3 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
BMP2
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of BMP-2 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
26.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP2-34928.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution containing 10 mM sodium citrate pH 3.5.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. Do not reconstitute in PBS or cell culture media.

Notes

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Alternate Names for Recombinant Human BMP-2 Protein

  • BDA2
  • BMP2
  • BMP-2
  • BMP-2A
  • BMP2ABone morphogenetic protein 2A
  • bone morphogenetic protein 2
  • SSFSC

Background

Bone morphogenetic protein 2 plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. Mature BMP-2 forms disulfide linked homodimers and heterodimers with BMP-7. BMP-2 induces cartilage and bone formation, and BMP-2/BMP-7 heterodimers are even more potent than BMP-2 homodimers. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine production, and is required for cardiac contractility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for BMP-2 Recombinant Protein (NBP2-34928)(2)

Reviews for BMP-2 Recombinant Protein (NBP2-34928) (0)

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FAQs for BMP-2 Recombinant Protein (NBP2-34928). (Showing 1 - 2 of 2 FAQs).

  1. I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?
    • Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.
  2. Can you protein fluoresence labeled BMP2 protein?
    • Unfortunately, we don't custom make any fluorophore conjugation to protein, but only making fluorophore-antibody conjugation in house. This is because proteins diverse a lot, with the different number of sites that can be conjugated with fluorophores and thus the efficiency and the successful rate cannot be determined.

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Bioinformatics

Gene Symbol BMP2
Uniprot