Reactivity | HuSpecies Glossary |
Applications | PAGE, Bioactivity |
Format | Carrier-Free |
Concentration | LYOPH |
Description | A homodimeric protein consisting of two 115 amino acid non-glycosylated polypeptide chains corresponding to BMP-2 Source: E. coli Uniprot ID: P12643 Amino Acid Sequence: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR |
Details of Functionality | BMP2 protein is fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/ml, corresponding to a specific activity of > 5.0 x 10^3 IU/mg. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | BMP2 |
Purity | >95%, by SDS-PAGE and HPLC |
Endotoxin Note | Less than 1 EU/ug of BMP-2 as determined by LAL method. |
Dilutions |
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Theoretical MW | 26.0 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer | Lyophilized from a 0.2 um filtered concentrated solution containing 10 mM sodium citrate pH 3.5. |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | >95%, by SDS-PAGE and HPLC |
Reconstitution Instructions | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. Do not reconstitute in PBS or cell culture media. |
Research Areas for BMP-2 Recombinant Protein (NBP2-34928)Find related products by research area.
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