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BMP-2 Antibody (9E10G12) - BSA Free

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Western Blot: BMP-2 Antibody (9E10G12) [NBP2-52422] - Western blot analysis using BMP2 mAb against human BMP2 (AA: 283-396) recombinant protein. (Expected MW is 38.7 kDa)
Western Blot: BMP-2 Antibody (9E10G12) [NBP2-52422] - Western blot analysis using BMP2 mAb against human BMP2 (AA: 283-396) recombinant protein. (Expected MW is 38.7 kDa)
Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody (9E10G12) [NBP2-52422] - Analysis of MCF-7 cells using BMP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Flow Cytometry: BMP-2 Antibody (9E10G12) [NBP2-52422] - Analysis of Hela cells using BMP2 mouse mAb (green) and negative control (red).
Western Blot: BMP-2 Antibody (9E10G12) [NBP2-52422] - Analysis using BMP2 mAb against HEK293 (1) and BMP2 (AA: 283-396)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody (9E10G12) [NBP2-52422] - Immunofluorescence analysis of Hela cells using BMP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
ELISA: BMP-2 Antibody (9E10G12) [NBP2-52422] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
9E10G12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-52422-0.025mg
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BMP-2 Antibody (9E10G12) - BSA Free Summary

Immunogen
Purified recombinant fragment of human BMP-2 (AA: 283-396) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BMP2
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Western Blot 1:500-1:2000
Theoretical MW
44.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for BMP-2 Antibody (9E10G12) - BSA Free

  • BDA2
  • BMP2
  • BMP-2
  • BMP-2A
  • BMP2ABone morphogenetic protein 2A
  • bone morphogenetic protein 2
  • SSFSC

Background

Bone morphogenic proteins (BMPs) are members of the TGFbeta superfamily. BMPs are involved in the induction of cartilage and bone formation. In vivo studies have shown that BMP-2 (also designated BMP-2A) and BMP-3 can independently induce cartilage formation. Smad3 association with the TGFbeta receptor complex and Smad1 translocation to the nucleus are observed after the addition of BMP-4 (also designated BMP-2B), suggesting that BMP-4 may play a role in activation of the Smad pathway. BMP-5, BMP-6 and BMP-7 all share high sequence homology with BMP-2, indicating that they each may be able to induce cartilage formation. BMP-8 (also designated OP-2) is thought to be involved in early development, as detectable expression has not been found in adult organs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BMP-2 Antibody (NBP2-52422). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?
    • Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.

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Bioinformatics

Gene Symbol BMP2