![Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against human Bmi1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Western-Blot-NBP2-22204-img0007.jpg "Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against human Bmi1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | 3E3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Purified recombinant fragment of human BMI-1 expressed in E. Coli. |
| Localization | Nucleus, Cytoplasm |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BMI1 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Ascites |
| Preservative | 0.03% Sodium Azide |
| Purity | Unpurified |
| Publications using NBP2-22204 | Applications | Species |
|---|---|---|
| Balakrishnan I, Danis E, Pierce A et al Senescence Induced by BMI1 Inhibition Is a Therapeutic Vulnerability in H3K27M-Mutant DIPG Cell Rep 2020-10-21 [PMID: 33086074] (IF/IHC, WB, WB, IF/IHC, Human) Details: Citation using the IHC-Prediluted version of this antibody. |
IF/IHC, WB, WB, IF/IHC | Human |
| RASHA M. RAMADAN M.D. Y, ISMAIL AMER M.D. S Immunohistochemical Expression of Leptin in Mammary Carcinoma The Medical Journal of Cairo University 2021-03-01 |
Secondary Antibodies |
Isotype Controls |
Research Areas for BMI-1 Antibody (NBP2-22204)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BMI1 |
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of Hela cells using Bmi1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunocytochemistry-Immunofluorescence-NBP2-22204-img0002.jpg "Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of Hela cells using Bmi1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded rectum cancer tissues using Bmi1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunohistochemistry-Paraffin-NBP2-22204-img0004.jpg "Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded rectum cancer tissues using Bmi1 mouse mAb with DAB staining.")
![Flow Cytometry: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of NIH/3T3 cells using Bmi1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Flow-Cytometry-NBP2-22204-img0001.jpg "Flow Cytometry: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of NIH/3T3 cells using Bmi1 mouse mAb (green) and negative control (red).")
![Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Western-Blot-NBP2-22204-img0006.jpg "Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded cervical cancer tissues using Bmi1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunohistochemistry-Paraffin-NBP2-22204-img0003.jpg "Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded cervical cancer tissues using Bmi1 mouse mAb with DAB staining.")
![ELISA: BMI-1 Antibody (3E3) [NBP2-22204] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-ELISA-NBP2-22204-img0005.jpg "ELISA: BMI-1 Antibody (3E3) [NBP2-22204] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")