![Western Blot: beta-Actin Antibody [HRP] [NB600-532H] - (c) GDF15 expression levels were analyzed with beta actin as an endogenous control, and are presented relative to those in BM cells. GDF15 protein levels were higher in CD34-positive than in CD34-negative BM cells. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36758) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-[HRP]-Western-Blot-NB600-532H-img0002.jpg "Western Blot: beta-Actin Antibody [HRP] [NB600-532H] - (c) GDF15 expression levels were analyzed with beta actin as an endogenous control, and are presented relative to those in BM cells. GDF15 protein levels were higher in CD34-positive than in CD34-negative BM cells. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36758) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, SyHa, Gp, Pm-Or, Rb, Sh, XpSpecies Glossary |
| Applications | ICC/IF (-), IP (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | HRP |
| Immunogen | This beta-Actin Antibody maps to a region corresponding to the N-terminus of human Beta Actin. [UniProt# P60709] |
| Predicted Species | Rat (100%), Porcine (100%), Chimpanzee (100%), Bovine (100%), Orangutan (100%), Guinea Pig (100%), Rabbit (100%), Chicken (100%), Canine (100%), Sheep (100%), Golden Syrian Hamster (100%), Xenopus (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ACTB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Application Notes | This antibody is useful for Western Blot. A 40 kDa band is detected in HeLa whole cell lysate and mouse NIH3T3 cells. This antibody is also useful for immunocytochemistry/immunofluorescence. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | human cancer cell line and human cancer cell lines | 05/19/2020 |
Summary
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Enlarge |
reviewed by:
Verified Customer |
WB | Human | 10/16/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
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The use of Beta Actin (AC-15) as a loading control across multiple species Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs. Actin has six highly conserved isoforms, however beta and gamma actin are the two... Read full blog post. |
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Tips on choosing an ideal loading control antibody for Western Blotting Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p... Read full blog post. |
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Beta-Actin's Role in Neuronal Plasticity Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro... Read full blog post. |
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ACTB - an abundant cytoskeletal component with applications for gene expression analysis Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi... Read full blog post. |
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A New Standard in Antibody Testing - Simple Western Certified Antibodies The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Verified Customer 05/19/2020 |
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| Application: | WB | |
| Species: | human cancer cell line and human cancer cell lines |
| Verified Customer 10/16/2014 |
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| Application: | WB | |
| Species: | Human |
| Gene Symbol | ACTB |
![Western Blot: beta-Actin Antibody [HRP] [NB600-532H] - Beta Actin Antibody [HRP] [NB600-532H] - Analysis of Beta Actin in MCF-7 cell lysate (20 ug) using anti-Beta Actin antibody. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Beta-Actin-Antibody-[HRP]-Western-Blot-NB600-532H-img0001.jpg "Western Blot: beta-Actin Antibody [HRP] [NB600-532H] - Beta Actin Antibody [HRP] [NB600-532H] - Analysis of Beta Actin in MCF-7 cell lysate (20 ug) using anti-Beta Actin antibody. WB image submitted by a verified customer review.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
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