| Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, SyHa, Gp, Pm-Or, Rb, Sh, XpSpecies Glossary |
| Applications | ICC/IF (-), IP (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | DyLight 488 |
| Description | This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet. |
| Immunogen | This beta-Actin Antibody maps to a region corresponding to the N-terminus of human Beta Actin. [UniProt# P60709] |
| Predicted Species | Rat (100%), Porcine (100%), Chimpanzee (100%), Bovine (100%), Orangutan (100%), Guinea Pig (100%), Rabbit (100%), Chicken (100%), Canine (100%), Sheep (100%), Golden Syrian Hamster (100%), Xenopus (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ACTB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Application Notes | This antibody is useful for Western Blot. A 40 kDa band is detected in HeLa whole cell lysate and mouse NIH3T3 cells. This antibody is also useful for immunocytochemistry/immunofluorescence. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ACTB |
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![Western Blot: beta-Actin Antibody [HRP] [NB600-532H] - (c) GDF15 expression levels were analyzed with beta actin as an endogenous control, and are presented relative to those in BM cells. GDF15 protein levels were higher in CD34-positive than in CD34-negative BM cells. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep36758) licensed under a CC-BY license.](https://images.novusbio.com/images/beta-Actin-Antibody-[HRP]-Western-Blot-NB600-532H-img0002.jpg "Western Blot: beta-Actin Antibody [HRP] [NB600-532H] - (c) GDF15 expression levels were analyzed with beta actin as an endogenous control, and are presented relative to those in BM cells. GDF15 protein levels were higher in CD34-positive than in CD34-negative BM cells. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep36758) licensed under a CC-BY license.")