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BCOR Antibody (CL13841)

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Analysis in human lymph node and pancreas tissues using NBP3-24581 antibody. Corresponding BCOR RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Analysis in human lymph node and pancreas tissues using NBP3-24581 antibody. Corresponding BCOR RNA-seq data are presented for the same tissues.
Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
Staining of human ovarian cancer (papillary carcinoma) shows strong nuclear positivity in tumor cells.
Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Staining of human cervical cancer shows strong nuclear positivity in tumor cells.
Staining in HEK293 cell line with Anti-BCOR monoclonal antibody, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Staining of human lymph node shows moderate nuclear positivity in non-germinal center cells.
Staining of human breast cancer (ductal carcinoma) shows strong nuclear positivity in tumor cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clone
CL13841
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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BCOR Antibody (CL13841) Summary

Immunogen
This antibody was designed to target the recombinant protein BCOR, with the precise immunogen sequence being withheld for proprietary reasons.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
BCOR
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for BCOR Antibody (CL13841)

  • ANOP2
  • BCL6 corepressor
  • BCL-6 corepressor
  • BCL6 co-repressor
  • BCL-6 interacting corepressor
  • BCoR
  • FLJ20285
  • FLJ38041
  • KIAA1575MGC71031
  • MAA2
  • MCOPS2
  • MGC131961

Background

BCOR is encoded by this gene was identified as an interacting corepressor of BCL6, a POZ/zinc finger transcription repressor that is required for germinal center formation and may influence apoptosis. This protein selectively interacts with the POZ domain of BCL6, but not with eight other POZ proteins. Specific class I and II histone deacetylases (HDACs) have been shown to interact with this protein, which suggests a possible link between the two classes of HDACs. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol BCOR