Bcl-2 Antibody

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Mouse, Rat, Canine.

We have publications tested in 5 applications: IF/IHC, IHC, IHC-P, WB, Western Blot.

Showing Publications 1 - 10 of 12. Show All 12 Publications. Collapse Publications.

Publications using NB100-56101	Applications	Species
Polat ME, Sar? E, Tanriverdi LH et al. A potential hepatoprotective effect of ranolazine against methotrexate-induced liver injury in rats Authorea 2023-01-01 (IHC, Rat)	IHC	Rat
Wu Z, Hu J, Li Y et al. Assessment of renal pathophysiological processes and protective effect of quercetin on contrast-induced acute kidney injury in type 1 diabetic mice using diffusion tensor imaging Redox Report : Communications in Free Radical Research 2024-09-16 [PMID: 39284588]
Debarpan Mitra, Depanwita Saha, Gaurav Das, Rimi Mukherjee, Samir Banerjee, Neyaz Alam, Saunak Mitra Mustafi, Partha Nath, Anuj Majumder, Biswanath Majumder, Nabendu Murmu Lupeol synergizes with 5-fluorouracil to combat c-MET/EphA2 mediated chemoresistance in triple negative breast cancer iScience 2023-11-04 [PMID: 38047085]
Arman T, Baron J, Lynch K Et al. MCLR-elicited hepatic fibrosis and carcinogenic gene expression changes persist in rats with diet-induced nonalcoholic steatohepatitis through a 4-week recovery period Toxicology 2021-11-01 [PMID: 34740672] (WB, Rat)	WB	Rat
Gao S, Zhou Q, Jin H et al. Effect of pyrroloquinoline quinone on lipopolysaccharide-induced autophagy in HAPI microglia cells Annals of Translational Medicine 2021-09-01 [PMID: 34733929] (Western Blot)	Western Blot
Zhou Q, Jin H, Shi N et al. Inhibit inflammation and apoptosis of pyrroloquinoline on spinal cord injury in rat Annals of Translational Medicine 2021-09-01 [PMID: 34733912]
Arman T, Lynch KD, Goedken M, Clarke JD Sub-chronic microcystin-LR renal toxicity in rats fed a high fat/high cholesterol diet Chemosphere 2020-10-27 [PMID: 33143886] (IHC-P, Rat)	IHC-P	Rat
Shoma Ishikawa, Fuyuki Ishikawa Proteostasis failure and cellular senescence in long‐term cultured postmitotic rat neurons Aging Cell 2019-11-25 [PMID: 31762159]
Sulaiman D, Li J, Devarajan A et al. Paraoxonase 2 protects against acute myocardial ischemia-reperfusion injury by modulating mitochondrial function and oxidative stress via the PI3K/Akt/GSK-3b RISK pathway J. Mol. Cell. Cardiol. 2019-02-23 [PMID: 30802459] (IHC-P, Mouse)	IHC-P	Mouse
Samdanci ET, Huz M, Ozhan O et al. Cytoprotective effects of molsidomine against methotrexate-induced hepatotoxicity: an experimental rat study Drug Des Devel Ther 2019-12-20 [PMID: 30587924] (IF/IHC, Rat)	IF/IHC	Rat
Rajamani U, Essop MF. Hyperglycemia-mediated activation of the hexosamine biosynthetic pathway results in myocardial apoptosis. Am J Physiol Cell Physiol. 2010-07-01 [PMID: 20410435]
Krajewska M, Rosenthal RE, Mikolajczyk J et al. Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation. Exp Neurol. 2004-10-01 [PMID: 15380478] (IHC-P, WB, Mouse, Canine, Rat) Details: Antibodies cited: 1. Caspase-9 (Active/Cleaved), IMG-5705: WB: Fig 1A (recombinant human caspase-9), Fig 4A (recombinant human caspase-9, rat brain), Fig 4B-F (isolated mitochondria from rat liver or heart). Fig 5 (PC12 cells) IHC (P): Fig 2d-k (rat kidne	IHC-P, WB	Mouse, Canine, Rat
Show All 12 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	3 reviewed by: Yuanyuan Tie	WB	Rat	11/23/2017	View Summary Application Western Blot Sample Tested H9c2 whole cell lysate Species Rat Lot AR09-092206B-05
Enlarge	5 reviewed by: Verified Customer	WB	Human	10/17/2014	View Summary Application Western Blot Sample Tested Whole cell lysate from 293T cells, in lysis buffer Species Human

Array NB100-56101

• Bcl-2 Antibodies
• Bcl-2 Antibody Pair
• Bcl-2 ELISA Kits
• Bcl-2 Proteins
• Bcl-2 ELISA
• Bcl-2 Proteins and Enzyme