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BBOX1 Antibody (OTI4F9)

Images

 
Western Blot: BBOX1 Antibody (OTI4F9) [NBP2-45548] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1.
Immunohistochemistry: BBOX1 Antibody (OTI4F9) [NBP2-45548] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BBOX1 Antibody (OTI4F9) [NBP2-45548] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BBOX1 Antibody (OTI4F9) [NBP2-45548] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescence: BBOX1 Antibody (OTI4F9) [NBP2-45548] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY BBOX1.
Immunofluorescence: BBOX1 Antibody (OTI4F9) [NBP2-45548] - Analysis of HeLa cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI4F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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BBOX1 Antibody (OTI4F9) Summary

Immunogen
Full length human recombinant protein of human BBOX1(NP_003977) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BBOX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:4000
Theoretical MW
44.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BBOX1 Antibody (OTI4F9)

  • 2-oxoglutarate dioxygenase 1
  • 2-oxoglutarate dioxygenase
  • BBHgamma-butyrobetaine
  • BBOXEC 1.14.11.1
  • butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetainehydroxylase) 1
  • Gamma-BBH
  • gamma-butyrobetaine dioxygenase
  • Gamma-butyrobetaine hydroxylase
  • Gamma-butyrobetaine
  • G-BBH

Background

BBOX1 encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BBOX1 Antibody (NBP2-45548) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BBOX1